Rapid detection method of alternariol in wheat flour based on nucleic acid adaptation

Rapid detection method of alternariol in wheat flour based on nucleic acid adaptation

The invention discloses a rapid detection method of alternariol in wheat flour based on nucleic acid adaptation. The method utilizes an aptamer probe to specifically capture alternariol, thereby effectively enhancing the detection stability and accuracy. The alternariol is a small molecular substanc...

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Hauptverfasser: WENG GUOWU, ZHU CHENLEI, LOU XINHUI
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator WENG GUOWU
ZHU CHENLEI
LOU XINHUI
description The invention discloses a rapid detection method of alternariol in wheat flour based on nucleic acid adaptation. The method utilizes an aptamer probe to specifically capture alternariol, thereby effectively enhancing the detection stability and accuracy. The alternariol is a small molecular substance, forms a competitive relationship with the nanogold after being combined with the aptamer, can bedistinguished by naked eyes by observing the color change of the solution based on the agglomeration condition of the nanogold; and using of a complex instrument is not needed, and on-site real-time detection is realized. The aptamer probe used in the method has the advantages of being capable of being artificially synthesized, convenient to modify, short in synthesis period, low in cost, good instability, small in batch-to-batch difference and capable of being stored for a long time, and can be stored after mass production, so that the detection period can be shortened. Meanwhile, visual rapid detection and semi-quan
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Rapid detection method of alternariol in wheat flour based on nucleic acid adaptation
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