Recombinant pichia pastoris engineering bacterium and application thereof to preparation of purine
The invention discloses recombinant pichia pastoris and an application thereof to preparation of purine. The recombinant pichia pastoris engineering bacterium is constructed by introducing a gene Rihc-2 shown in SEQ ID NO.1, a gene Hac1 shown in SEQ ID NO.2 and a gene PDI shown in SEQ ID NO.3 into h...
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| creator | WANG ZHAO WEI CHUN ZHAO LIXIANG SUN JIE QIAN XIAOFEN |
| description | The invention discloses recombinant pichia pastoris and an application thereof to preparation of purine. The recombinant pichia pastoris engineering bacterium is constructed by introducing a gene Rihc-2 shown in SEQ ID NO.1, a gene Hac1 shown in SEQ ID NO.2 and a gene PDI shown in SEQ ID NO.3 into host bacteria. According to the recombinant pichia pastoris and the application thereof to preparation of purine disclosed by the invention, adenosine hydrolase is efficiently expressed in pichia pastoris in a secretory expression manner for the first time, and protein expression enhancing strategiessuch as gene high copy, a resistance recyclable expression vector, HAC1 and PDI gene introduction and the like are combined and applied, so that the production efficiency of recombinase is improved.The strain WC-5 obtained by the invention adopts a fed-batch high-density fermentation method in a 5L fermentation tank, and the protein expression quantity of the adenosine hydrolase is 500mg/L. Thefermentation liquid superna |
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The recombinant pichia pastoris engineering bacterium is constructed by introducing a gene Rihc-2 shown in SEQ ID NO.1, a gene Hac1 shown in SEQ ID NO.2 and a gene PDI shown in SEQ ID NO.3 into host bacteria. According to the recombinant pichia pastoris and the application thereof to preparation of purine disclosed by the invention, adenosine hydrolase is efficiently expressed in pichia pastoris in a secretory expression manner for the first time, and protein expression enhancing strategiessuch as gene high copy, a resistance recyclable expression vector, HAC1 and PDI gene introduction and the like are combined and applied, so that the production efficiency of recombinase is improved.The strain WC-5 obtained by the invention adopts a fed-batch high-density fermentation method in a 5L fermentation tank, and the protein expression quantity of the adenosine hydrolase is 500mg/L. Thefermentation liquid superna</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201229&DB=EPODOC&CC=CN&NR=112143662A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25555,76308</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201229&DB=EPODOC&CC=CN&NR=112143662A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG ZHAO</creatorcontrib><creatorcontrib>WEI CHUN</creatorcontrib><creatorcontrib>ZHAO LIXIANG</creatorcontrib><creatorcontrib>SUN JIE</creatorcontrib><creatorcontrib>QIAN XIAOFEN</creatorcontrib><title>Recombinant pichia pastoris engineering bacterium and application thereof to preparation of purine</title><description>The invention discloses recombinant pichia pastoris and an application thereof to preparation of purine. The recombinant pichia pastoris engineering bacterium is constructed by introducing a gene Rihc-2 shown in SEQ ID NO.1, a gene Hac1 shown in SEQ ID NO.2 and a gene PDI shown in SEQ ID NO.3 into host bacteria. According to the recombinant pichia pastoris and the application thereof to preparation of purine disclosed by the invention, adenosine hydrolase is efficiently expressed in pichia pastoris in a secretory expression manner for the first time, and protein expression enhancing strategiessuch as gene high copy, a resistance recyclable expression vector, HAC1 and PDI gene introduction and the like are combined and applied, so that the production efficiency of recombinase is improved.The strain WC-5 obtained by the invention adopts a fed-batch high-density fermentation method in a 5L fermentation tank, and the protein expression quantity of the adenosine hydrolase is 500mg/L. 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The recombinant pichia pastoris engineering bacterium is constructed by introducing a gene Rihc-2 shown in SEQ ID NO.1, a gene Hac1 shown in SEQ ID NO.2 and a gene PDI shown in SEQ ID NO.3 into host bacteria. According to the recombinant pichia pastoris and the application thereof to preparation of purine disclosed by the invention, adenosine hydrolase is efficiently expressed in pichia pastoris in a secretory expression manner for the first time, and protein expression enhancing strategiessuch as gene high copy, a resistance recyclable expression vector, HAC1 and PDI gene introduction and the like are combined and applied, so that the production efficiency of recombinase is improved.The strain WC-5 obtained by the invention adopts a fed-batch high-density fermentation method in a 5L fermentation tank, and the protein expression quantity of the adenosine hydrolase is 500mg/L. Thefermentation liquid superna</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Recombinant pichia pastoris engineering bacterium and application thereof to preparation of purine |
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