Method for testing that SLC can maintain homeostasis of Leydig and muscle-like cells in adult testis
The invention discloses a method for testing that SLC can maintain homeostasis of Leydig and muscle-like cells in adult testis. Stem cells related to testicular seminiferous tubules of adult SD rats are called as Leydig stem cells (SLC) and can be differentiated into Leydig cells in vitro and in viv...
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| creator | WU YUE CHEN DAN ZHAO XINGYI CHEN HAOLIN JI MINPENG DUAN PING HUANG FU GUAN XIAOJU LIN HAN WEN XIN ZHAO XINGXING |
| description | The invention discloses a method for testing that SLC can maintain homeostasis of Leydig and muscle-like cells in adult testis. Stem cells related to testicular seminiferous tubules of adult SD rats are called as Leydig stem cells (SLC) and can be differentiated into Leydig cells in vitro and in vivo. Proliferation and differentiation of the SLC are regulated by locally generated factors, including PDGF family members PDGF-AA and PDGF-BB. Both members are able to stimulate proliferation of SLC, while only PDGF-AA is able to stimulate differentiation of SLC into Leydig cells producing testosterone (T), while PDGF-BB inhibits the process. In the method, it is verified that the SLC can actually form Leydig cells or myocyte-like cells under different differentiation conditions. PDGF-AA + LH can enable the SLC to be differentiated into Leydig cells capable of generating T, and PDGF-BB + TGFB induces the cells to become muscle-like cells for expressing smooth muscle actin. The method can effectively verify pluripot |
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Stem cells related to testicular seminiferous tubules of adult SD rats are called as Leydig stem cells (SLC) and can be differentiated into Leydig cells in vitro and in vivo. Proliferation and differentiation of the SLC are regulated by locally generated factors, including PDGF family members PDGF-AA and PDGF-BB. Both members are able to stimulate proliferation of SLC, while only PDGF-AA is able to stimulate differentiation of SLC into Leydig cells producing testosterone (T), while PDGF-BB inhibits the process. In the method, it is verified that the SLC can actually form Leydig cells or myocyte-like cells under different differentiation conditions. PDGF-AA + LH can enable the SLC to be differentiated into Leydig cells capable of generating T, and PDGF-BB + TGFB induces the cells to become muscle-like cells for expressing smooth muscle actin. 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Stem cells related to testicular seminiferous tubules of adult SD rats are called as Leydig stem cells (SLC) and can be differentiated into Leydig cells in vitro and in vivo. Proliferation and differentiation of the SLC are regulated by locally generated factors, including PDGF family members PDGF-AA and PDGF-BB. Both members are able to stimulate proliferation of SLC, while only PDGF-AA is able to stimulate differentiation of SLC into Leydig cells producing testosterone (T), while PDGF-BB inhibits the process. In the method, it is verified that the SLC can actually form Leydig cells or myocyte-like cells under different differentiation conditions. PDGF-AA + LH can enable the SLC to be differentiated into Leydig cells capable of generating T, and PDGF-BB + TGFB induces the cells to become muscle-like cells for expressing smooth muscle actin. 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Stem cells related to testicular seminiferous tubules of adult SD rats are called as Leydig stem cells (SLC) and can be differentiated into Leydig cells in vitro and in vivo. Proliferation and differentiation of the SLC are regulated by locally generated factors, including PDGF family members PDGF-AA and PDGF-BB. Both members are able to stimulate proliferation of SLC, while only PDGF-AA is able to stimulate differentiation of SLC into Leydig cells producing testosterone (T), while PDGF-BB inhibits the process. In the method, it is verified that the SLC can actually form Leydig cells or myocyte-like cells under different differentiation conditions. PDGF-AA + LH can enable the SLC to be differentiated into Leydig cells capable of generating T, and PDGF-BB + TGFB induces the cells to become muscle-like cells for expressing smooth muscle actin. The method can effectively verify pluripot</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | Method for testing that SLC can maintain homeostasis of Leydig and muscle-like cells in adult testis |
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