Method for reprogramming umbilical cord mesenchymal stem cells into liver cells and prepared liver organoids

Method for reprogramming umbilical cord mesenchymal stem cells into liver cells and prepared liver organoids

The invention discloses a method for reprogramming umbilical cord mesenchymal stem cells into liver cells. The method comprises the following steps of obtaining umbilical cord mesenchymal stem cells;carrying out pretreatment on the umbilical cord mesenchymal stem cells for two days; carrying out liv...

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Hauptverfasser: GAO YI, LIU FAN, WANG YIFENG, LI YANG, CHEN FENG, YI XIAO, GAO KUNJIE
Format: Patent
Sprache:chi ; eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator GAO YI
LIU FAN
WANG YIFENG
LI YANG
CHEN FENG
YI XIAO
GAO KUNJIE
description The invention discloses a method for reprogramming umbilical cord mesenchymal stem cells into liver cells. The method comprises the following steps of obtaining umbilical cord mesenchymal stem cells;carrying out pretreatment on the umbilical cord mesenchymal stem cells for two days; carrying out liver formation induction on the pretreated umbilical cord mesenchymal stem cells for seven days by using a liver formation induction composition; carrying out primary maturation induction on the umbilical cord mesenchymal stem cells subjected to liver formation induction treatment by using a first maturation induction composition for seven days to obtain liver cells; and carrying out second maturation induction on the liver cells for 12-20 days by using a second maturation induction composition to obtain the functional liver cells. Liver organoids comprise acellular matrix hydrogel, stem cells and liver cells, wherein the stem cells and liver cells being distributed in the acellular matrix hydrogel. According to the
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The method comprises the following steps of obtaining umbilical cord mesenchymal stem cells;carrying out pretreatment on the umbilical cord mesenchymal stem cells for two days; carrying out liver formation induction on the pretreated umbilical cord mesenchymal stem cells for seven days by using a liver formation induction composition; carrying out primary maturation induction on the umbilical cord mesenchymal stem cells subjected to liver formation induction treatment by using a first maturation induction composition for seven days to obtain liver cells; and carrying out second maturation induction on the liver cells for 12-20 days by using a second maturation induction composition to obtain the functional liver cells. Liver organoids comprise acellular matrix hydrogel, stem cells and liver cells, wherein the stem cells and liver cells being distributed in the acellular matrix hydrogel. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Method for reprogramming umbilical cord mesenchymal stem cells into liver cells and prepared liver organoids
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