Construction of recombinant pseudorabies virus vector expressing foreign protein and preparation method of recombinant pseudorabies virus
The invention provides a method for constructing pseudorabies virus replication non-essential gene insertion mutation by utilizing a CRISPR/Cas9 gene editing system and obtaining a recombinant pseudorabies virus expressing a foreign gene. After the CRISPR/Cas9 gene editing system is introduced into...
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| creator | SHANG YINGLI KONG ZHENGJIE LIU ZHEN |
| description | The invention provides a method for constructing pseudorabies virus replication non-essential gene insertion mutation by utilizing a CRISPR/Cas9 gene editing system and obtaining a recombinant pseudorabies virus expressing a foreign gene. After the CRISPR/Cas9 gene editing system is introduced into a cell, a viral genome is identified through a pre-screened target sequence, and a pseudorabies virus gene infecting the cell is edited and recombined. The recombinant pseudorabies virus constructed by the method is inserted into a target foreign gene at a specific gene part without affecting replication of the virus, and the recombinant virus is screened forwards or backwards by utilizing a marker in the construction process, so that the obtaining efficiency of the recombinant virus is remarkably improved, and a foundation is laid for constructing a recombinant pseudorabies virus vaccine expressing the foreign gene. The method for constructing the recombinant pseudorabies virus based on theCRISPR/Cas9 gene editing |
| format | Patent |
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After the CRISPR/Cas9 gene editing system is introduced into a cell, a viral genome is identified through a pre-screened target sequence, and a pseudorabies virus gene infecting the cell is edited and recombined. The recombinant pseudorabies virus constructed by the method is inserted into a target foreign gene at a specific gene part without affecting replication of the virus, and the recombinant virus is screened forwards or backwards by utilizing a marker in the construction process, so that the obtaining efficiency of the recombinant virus is remarkably improved, and a foundation is laid for constructing a recombinant pseudorabies virus vaccine expressing the foreign gene. 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After the CRISPR/Cas9 gene editing system is introduced into a cell, a viral genome is identified through a pre-screened target sequence, and a pseudorabies virus gene infecting the cell is edited and recombined. The recombinant pseudorabies virus constructed by the method is inserted into a target foreign gene at a specific gene part without affecting replication of the virus, and the recombinant virus is screened forwards or backwards by utilizing a marker in the construction process, so that the obtaining efficiency of the recombinant virus is remarkably improved, and a foundation is laid for constructing a recombinant pseudorabies virus vaccine expressing the foreign gene. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Construction of recombinant pseudorabies virus vector expressing foreign protein and preparation method of recombinant pseudorabies virus |
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