cffDNA aneuploid detection method and system, storage medium and detection equipment
The invention provides a cffDNA aneuploidy detection method and a system, a storage medium and detection equipment, and the method comprises the following steps: extracting cffDNA from a to-be-detected sample and constructing a library; sequencing the library to obtain sequencing data; according to...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | WU YINGSONG OUYANG GUOJUN WU LONG LI MING WENG RONGTAO YANG XUEXI |
| description | The invention provides a cffDNA aneuploidy detection method and a system, a storage medium and detection equipment, and the method comprises the following steps: extracting cffDNA from a to-be-detected sample and constructing a library; sequencing the library to obtain sequencing data; according to the sequencing data, obtaining a Zscore value corresponding to the target chromosome and double fetal concentrations; wherein the dual fetal concentration is used for reflecting the mutual relation between chromosome fetal concentration and standard fetal concentration; and respectively carrying outthreshold comparison on the Zscore value and the dual fetal concentration, and judging the aneuploidy of the target chromosome according to the threshold comparison result.
本发明提出了一种cffDNA非整倍体检测方法、系统、储存介质以及检测设备，其中，方法包括以下步骤：从待测样本中提取cffDNA并构建文库；对所述文库进行测序获取测序数据；根据所述测序数据获取目标染色体对应的Zscore值以及双重胎儿浓度；其中，所述双重胎儿浓度用于反映染色体胎儿浓度与标准胎儿浓度的相互关系；分别对所述Zscore值以及双重胎儿浓度进行阈值比较，根据所述阈值比较结果判断目标染色体的非整倍性。 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN112037846A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN112037846A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN112037846A3</originalsourceid><addsrcrecordid>eNrjZAhJTktz8XNUSMxLLS3Iyc9MUUhJLUlNLsnMz1PITS3JyE8BSqUoFFcWl6Tm6igUl-QXJaanAqVSMktzwVII9amFpZkFual5JTwMrGmJOcWpvFCam0HRzTXE2UM3tSA_PrW4IDE5NS-1JN7Zz9DQyMDY3MLEzNGYGDUAgFI4wg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>cffDNA aneuploid detection method and system, storage medium and detection equipment</title><source>esp@cenet</source><creator>WU YINGSONG ; OUYANG GUOJUN ; WU LONG ; LI MING ; WENG RONGTAO ; YANG XUEXI</creator><creatorcontrib>WU YINGSONG ; OUYANG GUOJUN ; WU LONG ; LI MING ; WENG RONGTAO ; YANG XUEXI</creatorcontrib><description>The invention provides a cffDNA aneuploidy detection method and a system, a storage medium and detection equipment, and the method comprises the following steps: extracting cffDNA from a to-be-detected sample and constructing a library; sequencing the library to obtain sequencing data; according to the sequencing data, obtaining a Zscore value corresponding to the target chromosome and double fetal concentrations; wherein the dual fetal concentration is used for reflecting the mutual relation between chromosome fetal concentration and standard fetal concentration; and respectively carrying outthreshold comparison on the Zscore value and the dual fetal concentration, and judging the aneuploidy of the target chromosome according to the threshold comparison result.
本发明提出了一种cffDNA非整倍体检测方法、系统、储存介质以及检测设备，其中，方法包括以下步骤：从待测样本中提取cffDNA并构建文库；对所述文库进行测序获取测序数据；根据所述测序数据获取目标染色体对应的Zscore值以及双重胎儿浓度；其中，所述双重胎儿浓度用于反映染色体胎儿浓度与标准胎儿浓度的相互关系；分别对所述Zscore值以及双重胎儿浓度进行阈值比较，根据所述阈值比较结果判断目标染色体的非整倍性。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201204&DB=EPODOC&CC=CN&NR=112037846A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201204&DB=EPODOC&CC=CN&NR=112037846A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WU YINGSONG</creatorcontrib><creatorcontrib>OUYANG GUOJUN</creatorcontrib><creatorcontrib>WU LONG</creatorcontrib><creatorcontrib>LI MING</creatorcontrib><creatorcontrib>WENG RONGTAO</creatorcontrib><creatorcontrib>YANG XUEXI</creatorcontrib><title>cffDNA aneuploid detection method and system, storage medium and detection equipment</title><description>The invention provides a cffDNA aneuploidy detection method and a system, a storage medium and detection equipment, and the method comprises the following steps: extracting cffDNA from a to-be-detected sample and constructing a library; sequencing the library to obtain sequencing data; according to the sequencing data, obtaining a Zscore value corresponding to the target chromosome and double fetal concentrations; wherein the dual fetal concentration is used for reflecting the mutual relation between chromosome fetal concentration and standard fetal concentration; and respectively carrying outthreshold comparison on the Zscore value and the dual fetal concentration, and judging the aneuploidy of the target chromosome according to the threshold comparison result.
本发明提出了一种cffDNA非整倍体检测方法、系统、储存介质以及检测设备，其中，方法包括以下步骤：从待测样本中提取cffDNA并构建文库；对所述文库进行测序获取测序数据；根据所述测序数据获取目标染色体对应的Zscore值以及双重胎儿浓度；其中，所述双重胎儿浓度用于反映染色体胎儿浓度与标准胎儿浓度的相互关系；分别对所述Zscore值以及双重胎儿浓度进行阈值比较，根据所述阈值比较结果判断目标染色体的非整倍性。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAhJTktz8XNUSMxLLS3Iyc9MUUhJLUlNLsnMz1PITS3JyE8BSqUoFFcWl6Tm6igUl-QXJaanAqVSMktzwVII9amFpZkFual5JTwMrGmJOcWpvFCam0HRzTXE2UM3tSA_PrW4IDE5NS-1JN7Zz9DQyMDY3MLEzNGYGDUAgFI4wg</recordid><startdate>20201204</startdate><enddate>20201204</enddate><creator>WU YINGSONG</creator><creator>OUYANG GUOJUN</creator><creator>WU LONG</creator><creator>LI MING</creator><creator>WENG RONGTAO</creator><creator>YANG XUEXI</creator><scope>EVB</scope></search><sort><creationdate>20201204</creationdate><title>cffDNA aneuploid detection method and system, storage medium and detection equipment</title><author>WU YINGSONG ; OUYANG GUOJUN ; WU LONG ; LI MING ; WENG RONGTAO ; YANG XUEXI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112037846A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WU YINGSONG</creatorcontrib><creatorcontrib>OUYANG GUOJUN</creatorcontrib><creatorcontrib>WU LONG</creatorcontrib><creatorcontrib>LI MING</creatorcontrib><creatorcontrib>WENG RONGTAO</creatorcontrib><creatorcontrib>YANG XUEXI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WU YINGSONG</au><au>OUYANG GUOJUN</au><au>WU LONG</au><au>LI MING</au><au>WENG RONGTAO</au><au>YANG XUEXI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>cffDNA aneuploid detection method and system, storage medium and detection equipment</title><date>2020-12-04</date><risdate>2020</risdate><abstract>The invention provides a cffDNA aneuploidy detection method and a system, a storage medium and detection equipment, and the method comprises the following steps: extracting cffDNA from a to-be-detected sample and constructing a library; sequencing the library to obtain sequencing data; according to the sequencing data, obtaining a Zscore value corresponding to the target chromosome and double fetal concentrations; wherein the dual fetal concentration is used for reflecting the mutual relation between chromosome fetal concentration and standard fetal concentration; and respectively carrying outthreshold comparison on the Zscore value and the dual fetal concentration, and judging the aneuploidy of the target chromosome according to the threshold comparison result.
本发明提出了一种cffDNA非整倍体检测方法、系统、储存介质以及检测设备，其中，方法包括以下步骤：从待测样本中提取cffDNA并构建文库；对所述文库进行测序获取测序数据；根据所述测序数据获取目标染色体对应的Zscore值以及双重胎儿浓度；其中，所述双重胎儿浓度用于反映染色体胎儿浓度与标准胎儿浓度的相互关系；分别对所述Zscore值以及双重胎儿浓度进行阈值比较，根据所述阈值比较结果判断目标染色体的非整倍性。</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN112037846A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | cffDNA aneuploid detection method and system, storage medium and detection equipment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T03%3A57%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=WU%20YINGSONG&rft.date=2020-12-04&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN112037846A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |