HPLC fingerprint detection method of liver-soothing stomach-harmonizing pain-relieving traditional Chinese medicine

The invention discloses a detection method of a fingerprint spectrum of a new traditional Chinese medicine, and particularly relates to an HPLC fingerprint spectrum detection method of a liver-soothing stomach-harmonizing pain-relieving traditional Chinese medicine. Aiming at the fingerprint spectrum of the liver-soothing stomach-harmonizing pain-relieving traditional Chinese medicine, conditionssuch as a chromatographic column, a mobile phase, a detection wavelength and a gradient elution program are optimized, liquid phase fingerprint spectrum determination conditions are established, methodological investigation is carried out, and the standard fingerprint spectrum of the liver-soothing stomach-harmonizing pain-relieving traditional Chinese medicine is established according to multiplebatches of pilot samples. According to the spectrum, 13 common peaks are determined in total, and medicinal material affiliation and component identification are carried out on the related common peaks. The quality of the liv