Production method of biosurfactant functional protein and culture medium used by production method
The invention discloses a production method of a biological surface active functional protein and a culture medium used by the production method. The invention provides a fungus fermentation medium which contains 0.1-1 g/L of corn steep liquor, 5-20 g/L of peptone and 20-40 g/L of lactose (or whey)....
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a production method of a biological surface active functional protein and a culture medium used by the production method. The invention provides a fungus fermentation medium which contains 0.1-1 g/L of corn steep liquor, 5-20 g/L of peptone and 20-40 g/L of lactose (or whey). The culture medium not only can be used for producing hydrophobin in trichoderma, but also can be used for producing other proteins in trichoderma. The filamentous fungi have similar culture conditions, so that the culture medium provided by the invention is also suitable for expression of hydrophobin or other proteins in other fungi. The fermentation process is simple and convenient, only a spore liquid needs to be inoculated into the fermentation culture medium, transfer is not needed, the raw materials are cheap, the cost is greatly reduced, and the method is suitable for industrial production and application.
本发明公开了一种生物表面活性功能蛋白的生产方法及其所用培养基。本发明提供了真菌发酵培养基,含有0.1-1g/L玉米浆、5-20g/L蛋白胨和20-40g/L乳糖(或乳清)。本发明培养基不仅可用于木霉中疏水 |
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