Molecular marker for identifying genetic sex of haliotis discus hannai and application thereof
The invention discloses a molecular marker for identifying genetic sex of haliotis discus hannai and an application of the molecular marker. The molecular marker is shown as insertion/deletion lengthpolymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual with the nucleotide sequen...
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| creator | CAI MINGYI LUO HANJIAO |
| description | The invention discloses a molecular marker for identifying genetic sex of haliotis discus hannai and an application of the molecular marker. The molecular marker is shown as insertion/deletion lengthpolymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual with the nucleotide sequence as shown in SEQ ID NO: 1 is expressed as genetic male haliotis discus hannai. During detection,SEQ ID NO: 2 and SEQ ID NO: 3 are adopted as primers for PCR amplification, and when the amplified fragments are 252bp and 240bp, the haliotis discus hannai to-be-detected lacks the gene of the nucleotide sequence shown in SEQ ID NO: 1 and is genetic male haliotis discus hannai; when only 252bp amplified fragments exist, the haliotis discus hannai to-be-detected has a nucleotide sequence shown asSEQ ID NO: 1 and is genetic female haliotis discus hannai. The molecular marker disclosed by the invention can be used for simply, conveniently, quickly and stably identifying the genetic sex of the haliotis discus hannai, is b |
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The molecular marker is shown as insertion/deletion lengthpolymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual with the nucleotide sequence as shown in SEQ ID NO: 1 is expressed as genetic male haliotis discus hannai. During detection,SEQ ID NO: 2 and SEQ ID NO: 3 are adopted as primers for PCR amplification, and when the amplified fragments are 252bp and 240bp, the haliotis discus hannai to-be-detected lacks the gene of the nucleotide sequence shown in SEQ ID NO: 1 and is genetic male haliotis discus hannai; when only 252bp amplified fragments exist, the haliotis discus hannai to-be-detected has a nucleotide sequence shown asSEQ ID NO: 1 and is genetic female haliotis discus hannai. The molecular marker disclosed by the invention can be used for simply, conveniently, quickly and stably identifying the genetic sex of the haliotis discus hannai, is b</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201009&DB=EPODOC&CC=CN&NR=111748639A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201009&DB=EPODOC&CC=CN&NR=111748639A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAI MINGYI</creatorcontrib><creatorcontrib>LUO HANJIAO</creatorcontrib><title>Molecular marker for identifying genetic sex of haliotis discus hannai and application thereof</title><description>The invention discloses a molecular marker for identifying genetic sex of haliotis discus hannai and an application of the molecular marker. The molecular marker is shown as insertion/deletion lengthpolymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual with the nucleotide sequence as shown in SEQ ID NO: 1 is expressed as genetic male haliotis discus hannai. During detection,SEQ ID NO: 2 and SEQ ID NO: 3 are adopted as primers for PCR amplification, and when the amplified fragments are 252bp and 240bp, the haliotis discus hannai to-be-detected lacks the gene of the nucleotide sequence shown in SEQ ID NO: 1 and is genetic male haliotis discus hannai; when only 252bp amplified fragments exist, the haliotis discus hannai to-be-detected has a nucleotide sequence shown asSEQ ID NO: 1 and is genetic female haliotis discus hannai. The molecular marker disclosed by the invention can be used for simply, conveniently, quickly and stably identifying the genetic sex of the haliotis discus hannai, is b</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEEKwjAQAHvxIOof1gd4CBW1RymKFz15tizJpl2MuyFJQX-vgg_wNAwMM61uZw1kx4AJHpjulMBrAnYkhf2LpYeehApbyPQE9TBgYC2cwXG2Y_64CDKgOMAYA1ssrAJloETq59XEY8i0-HFWLY-Ha3taUdSOckT7vXftxRizXe82dbOv_2neYms86w</recordid><startdate>20201009</startdate><enddate>20201009</enddate><creator>CAI MINGYI</creator><creator>LUO HANJIAO</creator><scope>EVB</scope></search><sort><creationdate>20201009</creationdate><title>Molecular marker for identifying genetic sex of haliotis discus hannai and application thereof</title><author>CAI MINGYI ; LUO HANJIAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN111748639A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CAI MINGYI</creatorcontrib><creatorcontrib>LUO HANJIAO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CAI MINGYI</au><au>LUO HANJIAO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Molecular marker for identifying genetic sex of haliotis discus hannai and application thereof</title><date>2020-10-09</date><risdate>2020</risdate><abstract>The invention discloses a molecular marker for identifying genetic sex of haliotis discus hannai and an application of the molecular marker. The molecular marker is shown as insertion/deletion lengthpolymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual with the nucleotide sequence as shown in SEQ ID NO: 1 is expressed as genetic male haliotis discus hannai. During detection,SEQ ID NO: 2 and SEQ ID NO: 3 are adopted as primers for PCR amplification, and when the amplified fragments are 252bp and 240bp, the haliotis discus hannai to-be-detected lacks the gene of the nucleotide sequence shown in SEQ ID NO: 1 and is genetic male haliotis discus hannai; when only 252bp amplified fragments exist, the haliotis discus hannai to-be-detected has a nucleotide sequence shown asSEQ ID NO: 1 and is genetic female haliotis discus hannai. The molecular marker disclosed by the invention can be used for simply, conveniently, quickly and stably identifying the genetic sex of the haliotis discus hannai, is b</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Molecular marker for identifying genetic sex of haliotis discus hannai and application thereof |
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