Screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot
The invention provides a screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot. The screening method comprises the following steps of: conducting plasmid construction, protein induction expression and protein separation and purification, conducting protein fluore...
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| creator | LI RUOHUI ZHANG YUAN FANG GUOKANG HUANG YINGCHUN |
| description | The invention provides a screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot. The screening method comprises the following steps of: conducting plasmid construction, protein induction expression and protein separation and purification, conducting protein fluorescence quenching reaction to screen a compound, and determining the optimal bacteria inhibition concentration of the compound. According to the screening method provided by the invention, through fluorescent spectrometry, a ligand molecule bound with the key protein of the Brassica pekinensis (Lour.) Rupr. soft rot is screened, and therefore, the medicine capable of treating the Brassica pekinensis (Lour.) Rupr. soft rot is obtained.
本发明提供一种用于治疗白菜软腐病的药物的筛选方法，包括如下步骤：质粒构建，蛋白诱导表达，蛋白分离纯化，蛋白的荧光淬灭反应筛选化合物，确定化合物的最佳抑菌浓度。本发明提供的方法通过荧光光谱法筛选与白菜软腐病菌关键蛋白结合的配体分子，从而得到可治疗白菜软腐病的药物。 |
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本发明提供一种用于治疗白菜软腐病的药物的筛选方法，包括如下步骤：质粒构建，蛋白诱导表达，蛋白分离纯化，蛋白的荧光淬灭反应筛选化合物，确定化合物的最佳抑菌浓度。本发明提供的方法通过荧光光谱法筛选与白菜软腐病菌关键蛋白结合的配体分子，从而得到可治疗白菜软腐病的药物。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200929&DB=EPODOC&CC=CN&NR=111718923A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200929&DB=EPODOC&CC=CN&NR=111718923A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI RUOHUI</creatorcontrib><creatorcontrib>ZHANG YUAN</creatorcontrib><creatorcontrib>FANG GUOKANG</creatorcontrib><creatorcontrib>HUANG YINGCHUN</creatorcontrib><title>Screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot</title><description>The invention provides a screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot. The screening method comprises the following steps of: conducting plasmid construction, protein induction expression and protein separation and purification, conducting protein fluorescence quenching reaction to screen a compound, and determining the optimal bacteria inhibition concentration of the compound. According to the screening method provided by the invention, through fluorescent spectrometry, a ligand molecule bound with the key protein of the Brassica pekinensis (Lour.) Rupr. soft rot is screened, and therefore, the medicine capable of treating the Brassica pekinensis (Lour.) Rupr. soft rot is obtained.
本发明提供一种用于治疗白菜软腐病的药物的筛选方法，包括如下步骤：质粒构建，蛋白诱导表达，蛋白分离纯化，蛋白的荧光淬灭反应筛选化合物，确定化合物的最佳抑菌浓度。本发明提供的方法通过荧光光谱法筛选与白菜软腐病菌关键蛋白结合的配体分子，从而得到可治疗白菜软腐病的药物。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEOAUEQxvFrFIJ3GB2FS9YVKLkQhShQaS6bvVk22NnMzL6_Ex5A9f3z5dcvrmfHiDHEG7xQ79SCJ-6yDS5EhCz4fZTR6kdt2IoEZyHhoxNRgsDkQJnLKZxy4hKEvAKTDouet0_B0W8HxXi3vdT7GSZqUJJ1GFGb-miMWZjlal6tq3_MG8lGOic</recordid><startdate>20200929</startdate><enddate>20200929</enddate><creator>LI RUOHUI</creator><creator>ZHANG YUAN</creator><creator>FANG GUOKANG</creator><creator>HUANG YINGCHUN</creator><scope>EVB</scope></search><sort><creationdate>20200929</creationdate><title>Screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot</title><author>LI RUOHUI ; ZHANG YUAN ; FANG GUOKANG ; HUANG YINGCHUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN111718923A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI RUOHUI</creatorcontrib><creatorcontrib>ZHANG YUAN</creatorcontrib><creatorcontrib>FANG GUOKANG</creatorcontrib><creatorcontrib>HUANG YINGCHUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI RUOHUI</au><au>ZHANG YUAN</au><au>FANG GUOKANG</au><au>HUANG YINGCHUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot</title><date>2020-09-29</date><risdate>2020</risdate><abstract>The invention provides a screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot. The screening method comprises the following steps of: conducting plasmid construction, protein induction expression and protein separation and purification, conducting protein fluorescence quenching reaction to screen a compound, and determining the optimal bacteria inhibition concentration of the compound. According to the screening method provided by the invention, through fluorescent spectrometry, a ligand molecule bound with the key protein of the Brassica pekinensis (Lour.) Rupr. soft rot is screened, and therefore, the medicine capable of treating the Brassica pekinensis (Lour.) Rupr. soft rot is obtained.
本发明提供一种用于治疗白菜软腐病的药物的筛选方法，包括如下步骤：质粒构建，蛋白诱导表达，蛋白分离纯化，蛋白的荧光淬灭反应筛选化合物，确定化合物的最佳抑菌浓度。本发明提供的方法通过荧光光谱法筛选与白菜软腐病菌关键蛋白结合的配体分子，从而得到可治疗白菜软腐病的药物。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Screening method for medicine used for treating Brassica pekinensis (Lour.) Rupr. soft rot |
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