DLase (dermatan sulfates lyase) and application thereof

The invention relates to DLase (dermatan sulfates lyase) and application thereof. The enzyme activity of the DLase provided by the invention for degrading dermatan sulfates is 800 to 1800 U/mg. The nucleotide sequence of a coded gene is as shown by SEQ ID NO.1 or SEQ ID NO.2, and the amino acid sequ...

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Bibliographische Detailangaben
Hauptverfasser:	JIAO RUNMIAO, LI FUCHUAN, HAN NAIHAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to DLase (dermatan sulfates lyase) and application thereof. The enzyme activity of the DLase provided by the invention for degrading dermatan sulfates is 800 to 1800 U/mg. The nucleotide sequence of a coded gene is as shown by SEQ ID NO.1 or SEQ ID NO.2, and the amino acid sequence is as shown by SEQ ID NO.3 or SEQ ID NO.4. The DLase related by the invention has higher dermatan sulfates degradation activity, and solves the problems that in the prior art, a specific degrading enzyme for the dermatan sulfates has low enzyme activity and is unfavorable for large-scale production; and meanwhile, higher temperature stability is realized. The DLase is an incision enzyme, and can be used for specific analysis of DS composition structures in CS/DS related samples, selective degradation removal of DS ingredients, preparation of DS oligosaccharides, quality control analysis of related products and the like. 本发明涉及硫酸皮肤素裂解酶及其应用。本发明中的硫酸皮肤素裂解酶DLase降解硫酸皮肤素的酶活力为800～1800U/mg，其编码基因核苷酸序列如SEQ ID NO.1或SEQ ID