Method for constructing reverse genetic system of red-spotted grouper nervous necrosis virus
The invention belongs to the technical field of virus gene engineering of aquatic animals, and particularly relates to a method for constructing a reverse genetic system of red-spotted grouper nervousnecrosis virus (RGNNV). According to the invention, CMV is used as a promoter to construct an eukary...
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| creator | TU JIAGANG ZHANG YONG'AN |
| description | The invention belongs to the technical field of virus gene engineering of aquatic animals, and particularly relates to a method for constructing a reverse genetic system of red-spotted grouper nervousnecrosis virus (RGNNV). According to the invention, CMV is used as a promoter to construct an eukaryotic expression plasmid containing an RGNNV genome; the RGNNV is rescued by adopting a method of combining human embryo kidney 293T cells with high transfection efficiency and striped snakehead cells (SSN-1) which efficiently replicate the RGNNV, so that the problem of low transfection efficiency of fish cells is solved.
本发明属于水生动物病毒基因工程技术领域，具体涉及一种构建赤点石斑鱼神经坏死病毒（red-spotted grouper nervous necrosis virus,RGNNV）反向遗传系统的方法。本发明以CMV为启动子构建含RGNNV基因组的真核表达质粒，采用高转染效率的人胚肾293T细胞和对RGNNV高效复制的条纹月鳢细胞（striped snakehead cell,SSN-1）组合的方法来拯救RGNNV，克服了鱼类细胞转染效率低的问题。 |
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本发明属于水生动物病毒基因工程技术领域，具体涉及一种构建赤点石斑鱼神经坏死病毒（red-spotted grouper nervous necrosis virus,RGNNV）反向遗传系统的方法。本发明以CMV为启动子构建含RGNNV基因组的真核表达质粒，采用高转染效率的人胚肾293T细胞和对RGNNV高效复制的条纹月鳢细胞（striped snakehead cell,SSN-1）组合的方法来拯救RGNNV，克服了鱼类细胞转染效率低的问题。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200731&DB=EPODOC&CC=CN&NR=111471710A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200731&DB=EPODOC&CC=CN&NR=111471710A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TU JIAGANG</creatorcontrib><creatorcontrib>ZHANG YONG'AN</creatorcontrib><title>Method for constructing reverse genetic system of red-spotted grouper nervous necrosis virus</title><description>The invention belongs to the technical field of virus gene engineering of aquatic animals, and particularly relates to a method for constructing a reverse genetic system of red-spotted grouper nervousnecrosis virus (RGNNV). According to the invention, CMV is used as a promoter to construct an eukaryotic expression plasmid containing an RGNNV genome; the RGNNV is rescued by adopting a method of combining human embryo kidney 293T cells with high transfection efficiency and striped snakehead cells (SSN-1) which efficiently replicate the RGNNV, so that the problem of low transfection efficiency of fish cells is solved.
本发明属于水生动物病毒基因工程技术领域，具体涉及一种构建赤点石斑鱼神经坏死病毒（red-spotted grouper nervous necrosis virus,RGNNV）反向遗传系统的方法。本发明以CMV为启动子构建含RGNNV基因组的真核表达质粒，采用高转染效率的人胚肾293T细胞和对RGNNV高效复制的条纹月鳢细胞（striped snakehead cell,SSN-1）组合的方法来拯救RGNNV，克服了鱼类细胞转染效率低的问题。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEKwkAQRdNYiHqH8QABF4XUEhQbrSyFEDY_cSHuLDOzAW9vBA9g9Yr3eMvicYU9uaOehTxHNcneQhxIMEEUNCDCgid9q-FF3M-mKzWxGToahHOCUIRMnHWmF9agNAXJui4WfTsqNj-uiu35dK8vJRI30NT677ypb865Q-Uqtzvu_2k-NGY80Q</recordid><startdate>20200731</startdate><enddate>20200731</enddate><creator>TU JIAGANG</creator><creator>ZHANG YONG'AN</creator><scope>EVB</scope></search><sort><creationdate>20200731</creationdate><title>Method for constructing reverse genetic system of red-spotted grouper nervous necrosis virus</title><author>TU JIAGANG ; ZHANG YONG'AN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN111471710A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TU JIAGANG</creatorcontrib><creatorcontrib>ZHANG YONG'AN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TU JIAGANG</au><au>ZHANG YONG'AN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for constructing reverse genetic system of red-spotted grouper nervous necrosis virus</title><date>2020-07-31</date><risdate>2020</risdate><abstract>The invention belongs to the technical field of virus gene engineering of aquatic animals, and particularly relates to a method for constructing a reverse genetic system of red-spotted grouper nervousnecrosis virus (RGNNV). According to the invention, CMV is used as a promoter to construct an eukaryotic expression plasmid containing an RGNNV genome; the RGNNV is rescued by adopting a method of combining human embryo kidney 293T cells with high transfection efficiency and striped snakehead cells (SSN-1) which efficiently replicate the RGNNV, so that the problem of low transfection efficiency of fish cells is solved.
本发明属于水生动物病毒基因工程技术领域，具体涉及一种构建赤点石斑鱼神经坏死病毒（red-spotted grouper nervous necrosis virus,RGNNV）反向遗传系统的方法。本发明以CMV为启动子构建含RGNNV基因组的真核表达质粒，采用高转染效率的人胚肾293T细胞和对RGNNV高效复制的条纹月鳢细胞（striped snakehead cell,SSN-1）组合的方法来拯救RGNNV，克服了鱼类细胞转染效率低的问题。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for constructing reverse genetic system of red-spotted grouper nervous necrosis virus |
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