Recombinant fibronectin mutant and application thereof

The invention belongs to the field of biology, and particularly relates to a nucleotide sequence of a recombinant fibronectin mutant and application of the recombinant fibronectin mutant. According tothe recombinant fibronectin mutant provided by the invention, a structural domain for promoting cell adhesion and cell proliferation on fibronectin is selected, the nucleotide sequence for coding therecombinant fibronectin mutant is designed by utilizing a translation pause theory, and under the condition of unchanging an amino acid sequence of the recombinant fibronectin, the recombinant fibronectin is obtained by replacing a codon with a higher translation speed in the last 20 codons of the recombinant fibronectin with a codon with a lower translation speed. The recombinant fibronectin mutant provided by the invention is simple in purification process and can promote cell proliferation activity and adhesion activity. 本发明属于生物学领域，具体涉及一种重组纤连蛋白突变体的核苷酸序列及其应用。本发明提供的重组纤连蛋白突变体，选择纤连蛋白上促细胞粘附、促细胞增殖结构域，并利用翻译暂停理论设计编码重组纤连蛋白突