Classical Swine fever virus and African swine fever virus dual-purpose one-step direct expansion real-time fluorescent quantitation RT-PCR detection reagent kit and application thereof

The invention discloses a classical Swine fever virus and African swine fever virus dual-purpose one-step direct expansion real-time fluorescent quantitation RT-PCR detection reagent kit and an application thereof. The reagent kit comprises a nucleic acid extracting solution, 2* Direct qRT-PCR Mix, enzyme mixed liquid, negative control, positive control and a primer and probe mixture, wherein thenucleotide sequences of primers are shown as SEQID NO.1 to SEQID NO.4, the nucleotide sequences of probes are shown as SEQID NO.5 and SEQID NO.6, and 5' ends of the probes as shown in SEQID NO.5 and SEQID NO.6 are respectively labelled with fluorescent report groups HEX and FAM; 3' ends of the probes are labelled with minor groove binders(MGB) and non-fluorescent quenchers(BHQ-1). The reagent kitcan realize quick identification and detection on classical Swine fever viruses and African swine fever viruses during one reaction, has the advantages of being high in specificity, good in stabilityand simple to operate, is n