Expression and purification method for peach receptor-like protein kinase

The invention discloses an expression and purification method for a peach receptor-like protein kinase. The method comprises the following steps: connecting intracellular domain cDNA of the receptor-like protein kinase with pET-28a(+) to obtain pET-28a(+)-FERONIA-KD, transforming the pET-28a(+)-FERONIA-KD into escherichia coli BL21(DE3) to obtain BL21-pET-28a-FERONIA-KD, optimizing the concentration of an inducer to improve the protein expression of the BL21-pET-28a-FERONIA-KD, and finally optimizing the pH of a washing buffer solution and an elution buffer solution used in a Ni-column purification process and an imidazole concentration to obtain high-concentration BL21-pET-28a-FERONIA-KD protein. The method reduces the difficulty of expression of the peach receptor-like protein kinase, and improves the expression of the protein and the purification effect of the protein. 本发明公开了桃的类受体蛋白激酶表达及纯化方法，将类受体蛋白激酶胞内域cDNA与pET-28a(+)连接，获得pET-28a(+)-FERONIA-KD，再将pET-28a(+)-FERONIA-KD转化到大肠杆菌BL21(DE3)中，获得BL21-pET-28a-FERONIA