Compositions, methods, kits and devices for molecular analysis

Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organicwater miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23 degrees centigrade. Also provided hereinare sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans. 本文提供了一种电泳分离介质，其包含：(a)非交联的或稀疏交联的聚合物或共聚物；(b)一种或多种变性剂化合物，其量足以抑制单链多核苷酸的复性；(c)水性溶剂；(d)可选地，适合于抑制电渗流的壁涂层材料；和(e)可选地，可与水混溶的有机溶剂，例如DMSO或乙腈，其中该电泳分离介质在23℃下显示至少七天的功能稳定性。