Nucleic acid detection kit for quantitative E. coli cell residues

The invention discloses a nucleic acid detection kit for quantitative E. coli cell residues. The nucleic acid detection kit can detect that the minimum concentration of the E. coli cell DNA is 0.1 fg/[mu]l, has excellent sensitivity and excellent specificity at the same time, and can be used to dete...

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Bibliographische Detailangaben
Hauptverfasser:	SUN MINGDI, WANG BOWEI, CHEN TAO, LI YING, CHANG ZISONG, LI TING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a nucleic acid detection kit for quantitative E. coli cell residues. The nucleic acid detection kit can detect that the minimum concentration of the E. coli cell DNA is 0.1 fg/[mu]l, has excellent sensitivity and excellent specificity at the same time, and can be used to detect the E. coli residual DNA in the samples of semi-finished products and finished products and the like of vaccine; and can provide reliable quality control evidence for production of a semi-finished product and a finished product and the like of the vaccine, and provide an important guarantee for safety production and use of the vaccine. 本发明公开了一种定量E.coli细胞残留的核酸检测试剂盒，本发明可以检测出E.coli细胞DNA的最低浓度为0.1fg/μl，在具有非常好的灵敏度同时还具有很好的特异性，可以用于检测疫苗半成品、成品等样品中E.coli细胞残留DNA；可为疫苗半成品、成品等生产环节提供等提供可靠的质控证据，为疫苗安全生产与使用提供重要保障。