Method for producing adenosine by fermentation
The invention belongs to the technical field of microbial fermentation and discloses a method for producing adenosine by fermentation. The method comprises the following steps: adenosine producing bacillus subtilis is taken as a fermentation strain, a fermentation tank containing a fermentation cult...
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| creator | BAO XIN ZHUANG HUIHUA ZHANG ZONGHUA YANG XIAOFANG SHI JINGDONG FENG SHIHONG LI JIANGLEI |
| description | The invention belongs to the technical field of microbial fermentation and discloses a method for producing adenosine by fermentation. The method comprises the following steps: adenosine producing bacillus subtilis is taken as a fermentation strain, a fermentation tank containing a fermentation culture medium is inoculated with a bacillus subtilis seed solution in an inoculation quantity of 5-15%,the culture temperature is 32-33 DEG C, the dissolved oxygen amount is 15-25%, the pH is controlled by automatically feeding ammonium hydroxide for 0-24 h, the pH is controlled at 6.8, and the pH iscontrolled at 6.5 from the 24 h to the end; a sucrose solution is fed to control the residual sugar content in a fermentation solution to be not lower than 1 g/L; and fermentation is carried out for 48-60 h in total to obtain an adenosine fermentation solution, wherein the fermentation culture medium comprises an oxygen carrier. A fermentation process is improved according to a biological metabolism regulation way of adeno |
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The method comprises the following steps: adenosine producing bacillus subtilis is taken as a fermentation strain, a fermentation tank containing a fermentation culture medium is inoculated with a bacillus subtilis seed solution in an inoculation quantity of 5-15%,the culture temperature is 32-33 DEG C, the dissolved oxygen amount is 15-25%, the pH is controlled by automatically feeding ammonium hydroxide for 0-24 h, the pH is controlled at 6.8, and the pH iscontrolled at 6.5 from the 24 h to the end; a sucrose solution is fed to control the residual sugar content in a fermentation solution to be not lower than 1 g/L; and fermentation is carried out for 48-60 h in total to obtain an adenosine fermentation solution, wherein the fermentation culture medium comprises an oxygen carrier. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for producing adenosine by fermentation |
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