Automatic analysis method and system for cyclic ribonucleic acid based on Nextflow

The embodiment of the invention provides an automatic analysis method and system for cyclic ribonucleic acid based on Nextflow, the system comprises a quality control module, a comparison module, a quantification module, a merging and deduplication module and a report generation module, and the function of each module is independently realized by one or more set software. According to the embodiment of the invention, analysis software of a plurality of cyclic ribonucleic acids is integrated through a Nextflow framework; and results analyzed by the plurality of pieces of software are compared,deduplicated and screened, and a final result is obtained by integrating the analysis results of different pieces of software, so that a more comprehensive and accurate cicRNA prediction analysis report can be obtained. 本发明实施例提供一种基于Nextflow的环状核糖核酸自动化分析方法及系统，所述系统包括质量控制模块、比对模块、定量模块、合并去重模块和报告生成模块，其中，每个模块的功能均由设定的一个或多个软件分别独立实现。本发明实施例通过Nextflow框架将多个环状核糖核酸的分析软件进行整合，并对所述多个软件分析出来的结果进行比对、去重和筛选，综合不同软件的分析结果得出最终结果，从而能够获得更加全面，精确的cicRNA