Kit and method for extracting DNA from rust-contaminated trace biological test materials

The invention provides a kit and method for extracting DNA from rust-contaminated trace biological test materials. A composition solution capable of efficiently removing rust from the rust-contaminated trace biological test materials is included, the composition solution is composed of hydroxy ethid...

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Bibliographische Detailangaben
Hauptverfasser: YAN ANXIN, HUANG LIWEI, ZHONG QIAOMEI, LI YONGJIU, TU ZHENG, ZHU CHUANHONG, CHEN JING, LUO BINGKE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a kit and method for extracting DNA from rust-contaminated trace biological test materials. A composition solution capable of efficiently removing rust from the rust-contaminated trace biological test materials is included, the composition solution is composed of hydroxy ethidine phosphonic acid (HEDP), sodium dodecyl sulfate (SDS), sarcosine, sodium dodecyl sarcosinate, cetyl trimethylammonium bromide (CTAB), cholic acid, deoxycholic acid, Benzoyl aminopropionic acid (BATC), octylphenol polyethoxylate, polyoxyethylene sorbitan monolaurate, tert-octylphenoxy poly(oxyethylene)ethanol, 1,4-piperazine bis-(ethanesulfonic acid), N-(2-acetylamino)-2-aminoethanesulfonic acid, polyethylene glycol-tert-octyl phenyl ether (Triton. RTM. X-100), (1,1,3,3-Tetramethylbutyl) phenyl-Polyethylene glycol (Triton. RTM. X-114) and water, the composition solution can be used as lysate releasing DNA to extract DNA in trace biological test materials in the kit box. 本发明提供一种从铁锈污染微量生物检材中提取DNA的试剂盒及方法,包括一种能够高效从铁锈