Overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as construction method and application of vector
The invention discloses an overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as a construction method and application of the vector. A bam gene is overexpressed in C.rhizophorae A761, and the nucleotide sequence of the bam gene is shown in SEQ ID NO.1. The method c...
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| creator | XU SHIHANG LI HAOHUA LIU TAOMEI LI SAINI ZHU MUZI CEN YOUFEI YE WEI KONG YALI ZHANG WEIMIN |
| description | The invention discloses an overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as a construction method and application of the vector. A bam gene is overexpressed in C.rhizophorae A761, and the nucleotide sequence of the bam gene is shown in SEQ ID NO.1. The method constructs the overexpression vector suitable for morinda officinalis endophytic fungi C.rhizophoraeA761 secondary metabolite biosynthesis genes for the first time, and provides a reliable molecular biological system for overexpression of secondary metabolic biosynthesis genes of other filamentous fungi in the later period, thereby laying the foundation for improving the yield of secondary metabolites of endophytic fungi and other filamentous fungi in a targeting manner; and at the same time, the method lays the foundation of molecular biology for later analysis of a biosynthesis mechanism of C.rhizophorae A761 cytosporaphenones compounds and discovery of novel cytosporaphenones compounds, and promotes the developme |
| format | Patent |
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A bam gene is overexpressed in C.rhizophorae A761, and the nucleotide sequence of the bam gene is shown in SEQ ID NO.1. The method constructs the overexpression vector suitable for morinda officinalis endophytic fungi C.rhizophoraeA761 secondary metabolite biosynthesis genes for the first time, and provides a reliable molecular biological system for overexpression of secondary metabolic biosynthesis genes of other filamentous fungi in the later period, thereby laying the foundation for improving the yield of secondary metabolites of endophytic fungi and other filamentous fungi in a targeting manner; and at the same time, the method lays the foundation of molecular biology for later analysis of a biosynthesis mechanism of C.rhizophorae A761 cytosporaphenones compounds and discovery of novel cytosporaphenones compounds, and promotes the developme</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200501&DB=EPODOC&CC=CN&NR=111088274A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76292</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200501&DB=EPODOC&CC=CN&NR=111088274A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XU SHIHANG</creatorcontrib><creatorcontrib>LI HAOHUA</creatorcontrib><creatorcontrib>LIU TAOMEI</creatorcontrib><creatorcontrib>LI SAINI</creatorcontrib><creatorcontrib>ZHU MUZI</creatorcontrib><creatorcontrib>CEN YOUFEI</creatorcontrib><creatorcontrib>YE WEI</creatorcontrib><creatorcontrib>KONG YALI</creatorcontrib><creatorcontrib>ZHANG WEIMIN</creatorcontrib><title>Overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as construction method and application of vector</title><description>The invention discloses an overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as a construction method and application of the vector. 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A bam gene is overexpressed in C.rhizophorae A761, and the nucleotide sequence of the bam gene is shown in SEQ ID NO.1. The method constructs the overexpression vector suitable for morinda officinalis endophytic fungi C.rhizophoraeA761 secondary metabolite biosynthesis genes for the first time, and provides a reliable molecular biological system for overexpression of secondary metabolic biosynthesis genes of other filamentous fungi in the later period, thereby laying the foundation for improving the yield of secondary metabolites of endophytic fungi and other filamentous fungi in a targeting manner; and at the same time, the method lays the foundation of molecular biology for later analysis of a biosynthesis mechanism of C.rhizophorae A761 cytosporaphenones compounds and discovery of novel cytosporaphenones compounds, and promotes the developme</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Overexpression vector suitable for morinda officinalis endophytic fungi A761 as well as construction method and application of vector |
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