Method for regulating and controlling components of polymyxin B homolog
The invention discloses a method for regulating and controlling the components of a polymyxin B homolog. The method comprises the following steps: (1) constructing pMAD-DeltaA and pMAD-DeltaC; (2) transforming pMAD-DeltaA into CJX518 strains, conducting screening to obtain successfully-transformed s...
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| creator | CHENG JINGSHENG YUAN YINGJIN YUAN YE |
| description | The invention discloses a method for regulating and controlling the components of a polymyxin B homolog. The method comprises the following steps: (1) constructing pMAD-DeltaA and pMAD-DeltaC; (2) transforming pMAD-DeltaA into CJX518 strains, conducting screening to obtain successfully-transformed strains, performing culturing and subculturing to obtain plasmid-lost strains, verifying the plasmid-lost strains as structural domain-replaced strains 518-A through PCR sequencing, transforming pMAD-DeltaC into the CJX518 strains, screening the pMAD-DeltaC transformed strains 518-A, carrying out culturing and subculturing to obtain plasmid-lost strains, and verifying the plasmid-lost strain as structural domain-replaced strains 518-AC through PCR sequencing; (3) carrying out seed culture to obtain secondary seeds; and (4) producing polymyxin B by inoculating the secondary seeds into a fermentation medium, and carrying out fermenting for 72 hours. According to the method disclosed by the invention, the components of |
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The method comprises the following steps: (1) constructing pMAD-DeltaA and pMAD-DeltaC; (2) transforming pMAD-DeltaA into CJX518 strains, conducting screening to obtain successfully-transformed strains, performing culturing and subculturing to obtain plasmid-lost strains, verifying the plasmid-lost strains as structural domain-replaced strains 518-A through PCR sequencing, transforming pMAD-DeltaC into the CJX518 strains, screening the pMAD-DeltaC transformed strains 518-A, carrying out culturing and subculturing to obtain plasmid-lost strains, and verifying the plasmid-lost strain as structural domain-replaced strains 518-AC through PCR sequencing; (3) carrying out seed culture to obtain secondary seeds; and (4) producing polymyxin B by inoculating the secondary seeds into a fermentation medium, and carrying out fermenting for 72 hours. According to the method disclosed by the invention, the components of</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200421&DB=EPODOC&CC=CN&NR=111041037A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200421&DB=EPODOC&CC=CN&NR=111041037A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHENG JINGSHENG</creatorcontrib><creatorcontrib>YUAN YINGJIN</creatorcontrib><creatorcontrib>YUAN YE</creatorcontrib><title>Method for regulating and controlling components of polymyxin B homolog</title><description>The invention discloses a method for regulating and controlling the components of a polymyxin B homolog. The method comprises the following steps: (1) constructing pMAD-DeltaA and pMAD-DeltaC; (2) transforming pMAD-DeltaA into CJX518 strains, conducting screening to obtain successfully-transformed strains, performing culturing and subculturing to obtain plasmid-lost strains, verifying the plasmid-lost strains as structural domain-replaced strains 518-A through PCR sequencing, transforming pMAD-DeltaC into the CJX518 strains, screening the pMAD-DeltaC transformed strains 518-A, carrying out culturing and subculturing to obtain plasmid-lost strains, and verifying the plasmid-lost strain as structural domain-replaced strains 518-AC through PCR sequencing; (3) carrying out seed culture to obtain secondary seeds; and (4) producing polymyxin B by inoculating the secondary seeds into a fermentation medium, and carrying out fermenting for 72 hours. 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The method comprises the following steps: (1) constructing pMAD-DeltaA and pMAD-DeltaC; (2) transforming pMAD-DeltaA into CJX518 strains, conducting screening to obtain successfully-transformed strains, performing culturing and subculturing to obtain plasmid-lost strains, verifying the plasmid-lost strains as structural domain-replaced strains 518-A through PCR sequencing, transforming pMAD-DeltaC into the CJX518 strains, screening the pMAD-DeltaC transformed strains 518-A, carrying out culturing and subculturing to obtain plasmid-lost strains, and verifying the plasmid-lost strain as structural domain-replaced strains 518-AC through PCR sequencing; (3) carrying out seed culture to obtain secondary seeds; and (4) producing polymyxin B by inoculating the secondary seeds into a fermentation medium, and carrying out fermenting for 72 hours. According to the method disclosed by the invention, the components of</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for regulating and controlling components of polymyxin B homolog |
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