TaqMan fluorescent quantitative PCR method for identifying pork components by utilizing CACA gene and application thereof
The invention discloses a TaqMan fluorescent quantitative PCR method for identifying pork components and an application thereof. Through a real-time fluorescent quantitative PCR technology based on aTaqMan probe, a single-copy gene CACA in pig cell nucleuses is used as a marker gene, and a pork comp...
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| creator | LIU TING LIU YAN FANG YING YAN YU'AO QIN RUI ZHANG LI WANG HAIYING |
| description | The invention discloses a TaqMan fluorescent quantitative PCR method for identifying pork components and an application thereof. Through a real-time fluorescent quantitative PCR technology based on aTaqMan probe, a single-copy gene CACA in pig cell nucleuses is used as a marker gene, and a pork component detection method with strong specificity and high sensitivity is established. The method established by the invention can be used for accurately distinguishing pigs from other 13 animals and 5 plants, carrying out actual detection on 6 kinds of meat products on the market and accurately judging whether the meat products contain pig-derived components or not. The pig-specific real-time fluorescent quantitative PCR detection method established by the invention can provide a technical reference for qualitative and quantitative detection of pig-derived components in mixed meat products.
本发明公开一种鉴定猪肉成分的TaqMan荧光定量PCR方法及其应用，通过基于TaqMan探针的实时荧光定量PCR技术，利用猪细胞核中的单拷贝基因CACA作为标记基因，建立具有特异性强、灵敏度高的猪肉成分检测的方法。本发明建立的方法可以准确区分猪与其他13种 |
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本发明公开一种鉴定猪肉成分的TaqMan荧光定量PCR方法及其应用，通过基于TaqMan探针的实时荧光定量PCR技术，利用猪细胞核中的单拷贝基因CACA作为标记基因，建立具有特异性强、灵敏度高的猪肉成分检测的方法。本发明建立的方法可以准确区分猪与其他13种</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200324&DB=EPODOC&CC=CN&NR=110904245A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200324&DB=EPODOC&CC=CN&NR=110904245A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU TING</creatorcontrib><creatorcontrib>LIU YAN</creatorcontrib><creatorcontrib>FANG YING</creatorcontrib><creatorcontrib>YAN YU'AO</creatorcontrib><creatorcontrib>QIN RUI</creatorcontrib><creatorcontrib>ZHANG LI</creatorcontrib><creatorcontrib>WANG HAIYING</creatorcontrib><title>TaqMan fluorescent quantitative PCR method for identifying pork components by utilizing CACA gene and application thereof</title><description>The invention discloses a TaqMan fluorescent quantitative PCR method for identifying pork components and an application thereof. Through a real-time fluorescent quantitative PCR technology based on aTaqMan probe, a single-copy gene CACA in pig cell nucleuses is used as a marker gene, and a pork component detection method with strong specificity and high sensitivity is established. The method established by the invention can be used for accurately distinguishing pigs from other 13 animals and 5 plants, carrying out actual detection on 6 kinds of meat products on the market and accurately judging whether the meat products contain pig-derived components or not. The pig-specific real-time fluorescent quantitative PCR detection method established by the invention can provide a technical reference for qualitative and quantitative detection of pig-derived components in mixed meat products.
本发明公开一种鉴定猪肉成分的TaqMan荧光定量PCR方法及其应用，通过基于TaqMan探针的实时荧光定量PCR技术，利用猪细胞核中的单拷贝基因CACA作为标记基因，建立具有特异性强、灵敏度高的猪肉成分检测的方法。本发明建立的方法可以准确区分猪与其他13种</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjkEKwjAQRbtxIeodxgMIrdaFyxIUN4pI9yW2k3YwzaRJKtTTG8EDuPrw_uPz58lUyuEiDSg9skNfowkwjNIECjLQC-Em7tBj6LgBxQ6oiQapiUwLlt0Tau4tmwg9PCYYA2l6f0tRiAJaNAjSNCCt1VTHRTYQOnTIapnMlNQeV79cJOvTsRTnDVqu0FsZv2CoxDXL0kOab_N9sfvH-QAZF0ad</recordid><startdate>20200324</startdate><enddate>20200324</enddate><creator>LIU TING</creator><creator>LIU YAN</creator><creator>FANG YING</creator><creator>YAN YU'AO</creator><creator>QIN RUI</creator><creator>ZHANG LI</creator><creator>WANG HAIYING</creator><scope>EVB</scope></search><sort><creationdate>20200324</creationdate><title>TaqMan fluorescent quantitative PCR method for identifying pork components by utilizing CACA gene and application thereof</title><author>LIU TING ; LIU YAN ; FANG YING ; YAN YU'AO ; QIN RUI ; ZHANG LI ; WANG HAIYING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN110904245A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU TING</creatorcontrib><creatorcontrib>LIU YAN</creatorcontrib><creatorcontrib>FANG YING</creatorcontrib><creatorcontrib>YAN YU'AO</creatorcontrib><creatorcontrib>QIN RUI</creatorcontrib><creatorcontrib>ZHANG LI</creatorcontrib><creatorcontrib>WANG HAIYING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU TING</au><au>LIU YAN</au><au>FANG YING</au><au>YAN YU'AO</au><au>QIN RUI</au><au>ZHANG LI</au><au>WANG HAIYING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TaqMan fluorescent quantitative PCR method for identifying pork components by utilizing CACA gene and application thereof</title><date>2020-03-24</date><risdate>2020</risdate><abstract>The invention discloses a TaqMan fluorescent quantitative PCR method for identifying pork components and an application thereof. Through a real-time fluorescent quantitative PCR technology based on aTaqMan probe, a single-copy gene CACA in pig cell nucleuses is used as a marker gene, and a pork component detection method with strong specificity and high sensitivity is established. The method established by the invention can be used for accurately distinguishing pigs from other 13 animals and 5 plants, carrying out actual detection on 6 kinds of meat products on the market and accurately judging whether the meat products contain pig-derived components or not. The pig-specific real-time fluorescent quantitative PCR detection method established by the invention can provide a technical reference for qualitative and quantitative detection of pig-derived components in mixed meat products.
本发明公开一种鉴定猪肉成分的TaqMan荧光定量PCR方法及其应用，通过基于TaqMan探针的实时荧光定量PCR技术，利用猪细胞核中的单拷贝基因CACA作为标记基因，建立具有特异性强、灵敏度高的猪肉成分检测的方法。本发明建立的方法可以准确区分猪与其他13种</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | TaqMan fluorescent quantitative PCR method for identifying pork components by utilizing CACA gene and application thereof |
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