Real-time fluorescent quantitative RT-PCR detection method for bovine norovirus
The invention relates to the technical field of molecular biology, in particular to a real-time fluorescent quantitative RT-PCR detection method for bovine norovirus (BNoV). According to the method, apair of specific primers is designed based on a targeted RdRp gene of the BNoV and a reaction system...
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| creator | MENG HONGLI SHI QIAOTING ZHANG BIN WANG YAZHOU LAN YALI SHI ZHIHAI XU ZHAOXUE ZHANG JIAQING |
| description | The invention relates to the technical field of molecular biology, in particular to a real-time fluorescent quantitative RT-PCR detection method for bovine norovirus (BNoV). According to the method, apair of specific primers is designed based on a targeted RdRp gene of the BNoV and a reaction system and reaction conditions of real-time fluorescent quantitative PCR are optimized, so that a fluorescent quantitative PCR method for detecting the BNoV is established. The method is good in linear relation and good in amplification efficiency; the sensitivity of the disclosed method is 1000 times ofthat of the conventional PCR method and is relatively high. The method only has a good amplification curve for BNoV, so that the specificity is high; and the difference in the groups and the difference between the groups are small and the high repeatability is realized.
本发明涉及分子生物学技术领域，具体涉及一种牛诺如病毒的实时荧光定量RT-PCR检测方法。该方法是基于BNoV的靶向RdRp基因设计一对特异性引物，并对实时荧光定量PCR的反应体系及反应条件进行优化，从而建立的检测BNoV的荧光定量PCR方法，该方法线性关系良好，扩增效率好；是常规PCR方法敏感性的1000倍 |
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本发明涉及分子生物学技术领域，具体涉及一种牛诺如病毒的实时荧光定量RT-PCR检测方法。该方法是基于BNoV的靶向RdRp基因设计一对特异性引物，并对实时荧光定量PCR的反应体系及反应条件进行优化，从而建立的检测BNoV的荧光定量PCR方法，该方法线性关系良好，扩增效率好；是常规PCR方法敏感性的1000倍</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200317&DB=EPODOC&CC=CN&NR=110885908A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200317&DB=EPODOC&CC=CN&NR=110885908A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MENG HONGLI</creatorcontrib><creatorcontrib>SHI QIAOTING</creatorcontrib><creatorcontrib>ZHANG BIN</creatorcontrib><creatorcontrib>WANG YAZHOU</creatorcontrib><creatorcontrib>LAN YALI</creatorcontrib><creatorcontrib>SHI ZHIHAI</creatorcontrib><creatorcontrib>XU ZHAOXUE</creatorcontrib><creatorcontrib>ZHANG JIAQING</creatorcontrib><title>Real-time fluorescent quantitative RT-PCR detection method for bovine norovirus</title><description>The invention relates to the technical field of molecular biology, in particular to a real-time fluorescent quantitative RT-PCR detection method for bovine norovirus (BNoV). According to the method, apair of specific primers is designed based on a targeted RdRp gene of the BNoV and a reaction system and reaction conditions of real-time fluorescent quantitative PCR are optimized, so that a fluorescent quantitative PCR method for detecting the BNoV is established. The method is good in linear relation and good in amplification efficiency; the sensitivity of the disclosed method is 1000 times ofthat of the conventional PCR method and is relatively high. The method only has a good amplification curve for BNoV, so that the specificity is high; and the difference in the groups and the difference between the groups are small and the high repeatability is realized.
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本发明涉及分子生物学技术领域，具体涉及一种牛诺如病毒的实时荧光定量RT-PCR检测方法。该方法是基于BNoV的靶向RdRp基因设计一对特异性引物，并对实时荧光定量PCR的反应体系及反应条件进行优化，从而建立的检测BNoV的荧光定量PCR方法，该方法线性关系良好，扩增效率好；是常规PCR方法敏感性的1000倍</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Real-time fluorescent quantitative RT-PCR detection method for bovine norovirus |
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