Mutant lipase with improved thermal stability and preparation method and application thereof

The invention discloses a mutant lipase with improved thermal stability and a preparation method and application thereof. The mutant lipase is obtained by introducing four pairs of disulfide-bond mutations into a Yarrowia lipolytica lipase 2 through an iterative combination method; and introduced four pairs of disulfide bonds are disulfide bonds S2-210, S8-214, S60-69 and S122-196, the obtained mutant lipase has high thermal stability and an enlarged pH reaction interval and thus is suitable for industrial application. An expression strain for increasing the yield of the mutant lipase is alsobuilt, the expression strain and molecular chaperones are co-expressed, and thus, the expression level of the mutant lipase can be further improved; and therefore, the thermal stability of the medium-temperature lipase can be greatly improved, at the same time, a high expression quantity is ensured, and powerful theoretical foundations are provided for industrial production. 本发明公开了一种热稳定性提高的突变脂肪酶及其制备方法与应用。该突变脂肪酶，是在耶氏解脂酵母脂肪