MiR-145-5P molecular marker for detecting type 2 diabetes mellitus, and amplification primers and application of miR-145-5P molecular marker

The invention discloses a miRNA (micro ribonucleic acid) molecular marker for detecting type 2 diabetes mellitus, and amplification primers and application of the miRNA molecular marker. The molecularmarker has the characteristics that a nucleotide sequence of an miR-145-5P molecular marker is 5...

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Bibliographische Detailangaben
Hauptverfasser:	XU XIAODONG, WANG SHANSHAN, LIU YANFEN, HUANG HUAYING, CHEN YINGTING, NIE DONGYING, LOU XUEYONG, LIU WENWEN, WANG XIAOQIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a miRNA (micro ribonucleic acid) molecular marker for detecting type 2 diabetes mellitus, and amplification primers and application of the miRNA molecular marker. The molecularmarker has the characteristics that a nucleotide sequence of an miR-145-5P molecular marker is 5'-GUCCAGUUUUCCCAGGAAUCCCU-3'; a nucleotide sequence of a forward amplification primer of the miR-145-5Pmolecular marker is 5'-GAGGGCACACCGTCGCGA-3'; and the molecular marker can be applied to a kit for detecting the type 2 diabetes mellitus. The molecular marker has the advantages that the molecular marker is high in detection efficiency and strong in pertinence and can be used for auxiliary diagnosis, detection or drug screening of the type 2 diabetes mellitus. 本发明公开了用于检测2型糖尿病的miRNA分子标记物及其扩增引物和应用，特点是分子标记物为miR-145-5P分子标志物，其核苷酸序列为5'-GUCCAGUUUUCCCAGGAAUCCCU-3'，其正向扩增引物的核苷酸序列为5'-GAGGGCACACCGTCGCGA-3'，可应用于检测2型糖尿病的试剂盒中，优点是检测效率高，针对性强，可用于2型糖尿病的辅助诊断、检测或筛查药物中。