RAA (recombinase aided amplification) constant temperature fluorescent detection primer probe set, kit and method for African swine fever virus CD2V gene
The invention belongs to the technical field of molecular biology and discloses an RAA (recombinase aided amplification) constant temperature fluorescent detection primer probe set, kit and method forAfrican swine fever virus CD2V gene. A nucleotide sequence of a forward primer of the primer probe s...
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Zusammenfassung: | The invention belongs to the technical field of molecular biology and discloses an RAA (recombinase aided amplification) constant temperature fluorescent detection primer probe set, kit and method forAfrican swine fever virus CD2V gene. A nucleotide sequence of a forward primer of the primer probe set is shown as SEQ ID NO. 1, a nucleotide sequence of a reverse primer is shown as SEQ ID NO. 2, and a nucleotide sequence of a specific fluorescent probe is shown as SEQ ID NO. 3, wherein a fluorescent reporter group is marked in the 5' end direction of the specific fluorescent probe, while a fluorescent quenching group is marked in the 3' end direction of the specific fluorescent probe. The primer probe set and kit provided by the invention help rapidly, conveniently, efficiently and specifically detect whether a sample is infected with an African swine fever wild strain or a CD2V deleted strain under an isothermal condition, do not need complex instruments and professional equipment on site, and are suitable for |
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