Chip colorimetric detection method based on light transmission result analysis
The invention discloses a chip colorimetric detection method based on light transmission result analysis. The method comprises a step of dropwise adding a reactant solution onto a chip carrier to obtain detection chips, a step of preparing standard detection substance solutions with different concen...
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| Hauptverfasser: | , , , , |
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| Format: | Patent |
| Sprache: | chi ; eng |
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| Zusammenfassung: | The invention discloses a chip colorimetric detection method based on light transmission result analysis. The method comprises a step of dropwise adding a reactant solution onto a chip carrier to obtain detection chips, a step of preparing standard detection substance solutions with different concentrations according to a concentration gradient, a step of dropwise adding the standard detection substance solutions with different concentrations into a detection area of a detection chip, developing color, measuring transmitted light and establishing a standard curve according to the relationshipbetween a transmitted light value and concentration values of the standard detection substance solutions to obtain a corresponding standard curve equation, and a step of dropwise adding a to-be-detected sample solution into the detection area of the detection chip, developing color, measuring transmission light, digitally reading a transmission light value of a to-be-detected sample, inputting thetransmission light value |
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