Preparation method of total alkaloids from rhizoma coptidis and application of total alkaloids from rhizoma coptidis in treatment of diabetes mellitus

The application provides a preparation method of total alkaloids from rhizoma coptidis. The preparation method includes the steps of ethanol heating reflux extraction, sodium chloride salting out andwater washing. The invention further provides application of a high-purity rhizoma coptidis biological total alkaloid product prepared by the method in preparation of drugs for the treatment of diabetes mellitus. The provided total alkaloids from the rhizoma coptidis with total alkaloids purity is 60% or above and berberine hydrochloride purity is 40% or above. The good effect is achieved when thediabetes mellitus is treated, and even a berberine pure product is surpassed. 本申请提供了一种包括乙醇加热回流提取、氯化钠盐析以及水洗步骤的黄连总碱制备方法，以及该方法制备的高纯度黄连生物总碱产品在制备治疗糖尿病的药物中的应用。提供的黄连总碱，总生物碱纯度达60％以上，盐酸小檗碱纯度达40％以上，在治疗糖尿病时具有良好的效果，甚至超过了小檗碱纯品。