Display substrate and preparation method thereof and display panel

The invention relates to the technical field of display, and discloses a display substrate and a preparation method thereof and a display panel. The display substrate comprises a flexible substrate. The flexible substrate comprises a bending region. A first groove is formed in the bending region. A first flexible layer is formed on the inner wall of at least the first groove. At least one convex rib is formed on the first flexible layer at the bottom of the first groove. A wiring layer covering the convex rib is formed on the first flexible layer, and the shape of alternating protrusions and depressions is formed at the place where the wiring layer covers the convex ribs and the positions of the protrusions are corresponding to the positions of the convex ribs. The shape of alternating protrusions and depressions is formed at the place where the wiring layer of the bending region covers the convex ribs. Compared with the planar wiring structure, the concave-convex alternating wiring structure can generate a l