Method for isolation and identification of bacillus circulans
The invention provides a method for isolation and identification of bacillus circulans, wherein the method comprises the following steps: (1) environmental bacteria containing bacillus circulans is subjected to selective enrichment culture by a selective enrichment culture medium, the proliferation...
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| creator | ZHANG CHEN WANG XIAOLI XU NING DU JUAN LIU XIU ZHU CHENCHEN LYU WEI WANG DI FENG SHIYANG SUN JIANNA CHEN BIXIAO WANG CAN LIU YANRONG YANG TINGTING LIU HONG WEN BOQIN DU YAQIONG YANG MIAO YANG YANG ZHOU FAN HAN BING ZHANG CHI LI YUANYUAN QIAO LING |
| description | The invention provides a method for isolation and identification of bacillus circulans, wherein the method comprises the following steps: (1) environmental bacteria containing bacillus circulans is subjected to selective enrichment culture by a selective enrichment culture medium, the proliferation of non-bacillus circulans is reduced, and the bacillus circulans is highly efficiently proliferates,wherein the selective enrichment culture medium is a modified tryptone soybean broth culture medium containing cephalosporin antibiotics and benzylpenicillin potassium; and (2) the bacterial solutionobtained in the step (1) is cultured in a Columbia blood agar plate containing antibiotics, and the strains having hemolytic ring appearing are isolated and are identified according to the colony morphology. The method can further use the molecular biological method to identify the strains isolated in the step (2). The method can simply and quickly isolate and identify bacillus circulans from theenvironmental bacteria, an |
| format | Patent |
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The method can further use the molecular biological method to identify the strains isolated in the step (2). The method can simply and quickly isolate and identify bacillus circulans from theenvironmental bacteria, an</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20191119&DB=EPODOC&CC=CN&NR=110468067A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20191119&DB=EPODOC&CC=CN&NR=110468067A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG CHEN</creatorcontrib><creatorcontrib>WANG XIAOLI</creatorcontrib><creatorcontrib>XU NING</creatorcontrib><creatorcontrib>DU JUAN</creatorcontrib><creatorcontrib>LIU XIU</creatorcontrib><creatorcontrib>ZHU CHENCHEN</creatorcontrib><creatorcontrib>LYU WEI</creatorcontrib><creatorcontrib>WANG DI</creatorcontrib><creatorcontrib>FENG SHIYANG</creatorcontrib><creatorcontrib>SUN JIANNA</creatorcontrib><creatorcontrib>CHEN BIXIAO</creatorcontrib><creatorcontrib>WANG CAN</creatorcontrib><creatorcontrib>LIU YANRONG</creatorcontrib><creatorcontrib>YANG TINGTING</creatorcontrib><creatorcontrib>LIU HONG</creatorcontrib><creatorcontrib>WEN BOQIN</creatorcontrib><creatorcontrib>DU YAQIONG</creatorcontrib><creatorcontrib>YANG MIAO</creatorcontrib><creatorcontrib>YANG YANG</creatorcontrib><creatorcontrib>ZHOU FAN</creatorcontrib><creatorcontrib>HAN BING</creatorcontrib><creatorcontrib>ZHANG CHI</creatorcontrib><creatorcontrib>LI YUANYUAN</creatorcontrib><creatorcontrib>QIAO LING</creatorcontrib><title>Method for isolation and identification of bacillus circulans</title><description>The invention provides a method for isolation and identification of bacillus circulans, wherein the method comprises the following steps: (1) environmental bacteria containing bacillus circulans is subjected to selective enrichment culture by a selective enrichment culture medium, the proliferation of non-bacillus circulans is reduced, and the bacillus circulans is highly efficiently proliferates,wherein the selective enrichment culture medium is a modified tryptone soybean broth culture medium containing cephalosporin antibiotics and benzylpenicillin potassium; and (2) the bacterial solutionobtained in the step (1) is cultured in a Columbia blood agar plate containing antibiotics, and the strains having hemolytic ring appearing are isolated and are identified according to the colony morphology. The method can further use the molecular biological method to identify the strains isolated in the step (2). 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and (2) the bacterial solutionobtained in the step (1) is cultured in a Columbia blood agar plate containing antibiotics, and the strains having hemolytic ring appearing are isolated and are identified according to the colony morphology. The method can further use the molecular biological method to identify the strains isolated in the step (2). The method can simply and quickly isolate and identify bacillus circulans from theenvironmental bacteria, an</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for isolation and identification of bacillus circulans |
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