Nitric oxide donor micromolecule, and preparation method and application thereof

The invention discloses a donor micromolecule, and a preparation method and application thereof. The donor micromolecule contains a nitroreductase trigger group and a novel nitric oxide donor (N-hydroxyguanidine), and has the advantages of simple synthesis process and efficient and rapid application. The donor micromolecule can release high-concentration nitric oxide under trigger of over-expressed nitroreductase in mitochondria of tumor cells to kill cancer cells. The nitric oxide donor introduced into the small molecule is applied to biological research for the first time, and the inventionprovides a new tool for developing a new nitric oxide release system and inhibiting activity of tumor cells. 本发明公开了一种供体小分子及其制备方法与应用，供体小分子中含有硝基还原酶触发基团和新型一氧化氮供体(N-羟基胍)，合成过程简单，应用高效、快捷。它可以在肿瘤细胞线粒体中过表达的硝基还原酶的触发下释放高浓度一氧化氮，使癌细胞凋亡。该小分子中所引入的一氧化氮供体是首次被应用于生物学研究，为开发新的一氧化氮释放系统和肿瘤细胞活性抑制提供了一种新工具。