Method for simultaneously measuring residual content of glyphosate and (aminomethyl)phosphonic acid in soil

The invention discloses a method for simultaneously determining residual content of glyphosate and (aminomethyl)phosphonic acid in soil. The method comprises the steps of adding an alkali aqueous solution into a soil sample to be measured for extracting; after extraction, adding an organic solvent into crude extract for purification; then adding a derivatization reagent to perform a derivatizationreaction, and thus acquiring liquid to be measured; and at last, measuring the concentrations of glyphosate and (aminomethyl)phosphonic acid derivatives by using a gradient elution-high performance liquid chromatography-fluorescence detector method. The method is simple, convenient, rapid and accurate to operate, small in relative mean variation; and the measurement precision and the detectable concentration can meet the requirements of soil environment monitoring and agroecological protection. 本发明公开了一种同时测定土壤草甘膦及氨甲基膦酸残余含量的方法，该方法是在待测土壤样品中加入碱性水溶液提取，提取后在粗提液中加入有机溶剂进行净化处理，而后加入衍生化试剂进行衍生化反应得到待测液，最后采用梯度淋洗-高效液相色谱-荧光检测器法测定草甘膦及