Method for preparing infectious bronchitis viral antigen by adopting non-immune eggs

Method for preparing infectious bronchitis viral antigen by adopting non-immune eggs

The invention belongs to the technical field of vaccine antigens, and discloses a method for preparing infectious bronchitis viral antigen by adopting non-immune eggs. The method comprises the following steps that infectious bronchitis non-immune eggs are adopted, pre-incubation conditions are contr...

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Hauptverfasser: JI XINGYU, LI BING, LI HOUWEI, MENG LEI, PENG LI, MENG XIANG, YAO FENMAO, ZHANG NING, ZHANG PANPAN, WANG YANHUI
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Sprache:chi ; eng
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HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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creator JI XINGYU
LI BING
LI HOUWEI
MENG LEI
PENG LI
MENG XIANG
YAO FENMAO
ZHANG NING
ZHANG PANPAN
WANG YANHUI
description The invention belongs to the technical field of vaccine antigens, and discloses a method for preparing infectious bronchitis viral antigen by adopting non-immune eggs. The method comprises the following steps that infectious bronchitis non-immune eggs are adopted, pre-incubation conditions are controlled for pre-incubation; toxic strains of infectious bronchitis virus are inoculated on a chick embryo, and the inoculation dose is 105.0EID50/piece; after inoculation, post-incubation conditions are controlled for post-incubation, inspecting is still conducted after inoculation for 24 h, a dead embryo is discarded, after inoculation for 36 h, the chick embryo is placed into a cold storage chamber at 2-8 DEG C, and a virus allantoic fluid is obtained after 12 hours of cold storage; the virus titer of the obtained virus allantoic fluid is detected, and the virus allantoic fluid is concentrated and purified to obtain the infectious bronchitis viral antigen. According to the method, SPF eggs are replaced by the non-i
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subjects HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
title Method for preparing infectious bronchitis viral antigen by adopting non-immune eggs
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