一种功能性全元生物肥料及其制备方法

本发明公开了一种功能性全元生物肥料及其制备方法,以秸秆和小龙虾加工废弃物为主要原料,经出芽短梗霉和芽孢杆菌发酵,即得;制备方法包括如下具体步骤:步骤1,将出芽短梗霉在马铃薯葡萄糖琼脂培养基中活化培养5d,芽孢杆菌在LB培养基中活化培养24 h;步骤2,将培养至对数生长期的出芽短梗霉和芽孢杆菌按照2:5~5:2进行混合;步骤3,按体积比4-20%的接种量取混菌体系种子液分别接入到含有秸秆的发酵培养基和含有小龙虾加工废弃物的发酵培养基中,28℃发酵培养2-10 d;步骤4,将步骤3发酵后的混菌体系接种至秸秆和小龙虾加工废弃物的混合物中,发酵制备全元生物有机肥料;其中,每吨混合物加入40-100Kg...

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description 本发明公开了一种功能性全元生物肥料及其制备方法,以秸秆和小龙虾加工废弃物为主要原料,经出芽短梗霉和芽孢杆菌发酵,即得;制备方法包括如下具体步骤:步骤1,将出芽短梗霉在马铃薯葡萄糖琼脂培养基中活化培养5d,芽孢杆菌在LB培养基中活化培养24 h;步骤2,将培养至对数生长期的出芽短梗霉和芽孢杆菌按照2:5~5:2进行混合;步骤3,按体积比4-20%的接种量取混菌体系种子液分别接入到含有秸秆的发酵培养基和含有小龙虾加工废弃物的发酵培养基中,28℃发酵培养2-10 d;步骤4,将步骤3发酵后的混菌体系接种至秸秆和小龙虾加工废弃物的混合物中,发酵制备全元生物有机肥料;其中,每吨混合物加入40-100Kg步骤3发酵后的混菌体系。
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subjects CHEMISTRY
FERTILISERS
FERTILISERS CHARACTERISED BY THEIR FORM
MANUFACTURE THEREOF
METALLURGY
MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05
MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS
ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
title 一种功能性全元生物肥料及其制备方法
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