Recombinant genetic engineering bacterium and application thereof in production of vanillin by fermentation
The invention discloses a recombinant genetic engineering bacterium and an application thereof in production of vanillin by fermentation. The recombinant genetic engineering bacterium is constructed by introducing a gene tolerant to vanillin or ferulic acid into a host bacterium. The gene tolerant t...
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| creator | LIU CHANGDONG YAN HUAYUAN ZHENG WANGJIAN CHEN XIAOLONG JIA XIAOQIN HOU QIANGBO |
| description | The invention discloses a recombinant genetic engineering bacterium and an application thereof in production of vanillin by fermentation. The recombinant genetic engineering bacterium is constructed by introducing a gene tolerant to vanillin or ferulic acid into a host bacterium. The gene tolerant to vanillin or ferulic acid includes one or more of a periplasmic membrane fusion protein AcrA gene, an inner membrane transport protein AcrB gene or an outer membrane protein TolC gene; the host includes escherichia coli containing an ech gene and an fcs gene. The recombinant genetic engineering bacterium E.coli BW25113::PegYB::pACYCDuet-acrAB-tolC can produce vanillin with ferulic acid as a substrate, and the maximum yield of vanillin is 2335 mg/L. A fermentation broth produced by fermentation by the method contains higher vanillin and good application prospect is achieved.
本发明公开了一种重组基因工程菌及其发酵产香兰素的应用，所述重组基因工程菌是由耐受香兰素或阿魏酸的基因导入宿主菌构建而成，所述耐受香兰素或阿魏酸的基因包括周质膜融合蛋白AcrA基因、内膜转运蛋白AcrB基因或外膜蛋白TolC基因中的一种或多种；所述宿主包括含ech基因和fcs基因的大肠 |
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本发明公开了一种重组基因工程菌及其发酵产香兰素的应用，所述重组基因工程菌是由耐受香兰素或阿魏酸的基因导入宿主菌构建而成，所述耐受香兰素或阿魏酸的基因包括周质膜融合蛋白AcrA基因、内膜转运蛋白AcrB基因或外膜蛋白TolC基因中的一种或多种；所述宿主包括含ech基因和fcs基因的大肠</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190920&DB=EPODOC&CC=CN&NR=110257312A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190920&DB=EPODOC&CC=CN&NR=110257312A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU CHANGDONG</creatorcontrib><creatorcontrib>YAN HUAYUAN</creatorcontrib><creatorcontrib>ZHENG WANGJIAN</creatorcontrib><creatorcontrib>CHEN XIAOLONG</creatorcontrib><creatorcontrib>JIA XIAOQIN</creatorcontrib><creatorcontrib>HOU QIANGBO</creatorcontrib><title>Recombinant genetic engineering bacterium and application thereof in production of vanillin by fermentation</title><description>The invention discloses a recombinant genetic engineering bacterium and an application thereof in production of vanillin by fermentation. The recombinant genetic engineering bacterium is constructed by introducing a gene tolerant to vanillin or ferulic acid into a host bacterium. The gene tolerant to vanillin or ferulic acid includes one or more of a periplasmic membrane fusion protein AcrA gene, an inner membrane transport protein AcrB gene or an outer membrane protein TolC gene; the host includes escherichia coli containing an ech gene and an fcs gene. The recombinant genetic engineering bacterium E.coli BW25113::PegYB::pACYCDuet-acrAB-tolC can produce vanillin with ferulic acid as a substrate, and the maximum yield of vanillin is 2335 mg/L. A fermentation broth produced by fermentation by the method contains higher vanillin and good application prospect is achieved.
本发明公开了一种重组基因工程菌及其发酵产香兰素的应用，所述重组基因工程菌是由耐受香兰素或阿魏酸的基因导入宿主菌构建而成，所述耐受香兰素或阿魏酸的基因包括周质膜融合蛋白AcrA基因、内膜转运蛋白AcrB基因或外膜蛋白TolC基因中的一种或多种；所述宿主包括含ech基因和fcs基因的大肠</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTEKwkAURNNYiHqH7wEEkyDWEhQrC7EPP5tJ8nHzd9lsBG9vDB7AaobHG2aZPO8wrq9EWSO1UEQxBG1FgSDaUsUmTm3sibUm9t6K4ShOKXYIcA2Jkg-uHs1MJ_BiFWsnXL2pQeihcV6sk0XDdsDml6tkezk_iusO3pUYPJvvf1nc0nSfHY55mp3yf5wPVyFCQQ</recordid><startdate>20190920</startdate><enddate>20190920</enddate><creator>LIU CHANGDONG</creator><creator>YAN HUAYUAN</creator><creator>ZHENG WANGJIAN</creator><creator>CHEN XIAOLONG</creator><creator>JIA XIAOQIN</creator><creator>HOU QIANGBO</creator><scope>EVB</scope></search><sort><creationdate>20190920</creationdate><title>Recombinant genetic engineering bacterium and application thereof in production of vanillin by fermentation</title><author>LIU CHANGDONG ; YAN HUAYUAN ; ZHENG WANGJIAN ; CHEN XIAOLONG ; JIA XIAOQIN ; HOU QIANGBO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN110257312A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU CHANGDONG</creatorcontrib><creatorcontrib>YAN HUAYUAN</creatorcontrib><creatorcontrib>ZHENG WANGJIAN</creatorcontrib><creatorcontrib>CHEN XIAOLONG</creatorcontrib><creatorcontrib>JIA XIAOQIN</creatorcontrib><creatorcontrib>HOU QIANGBO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU CHANGDONG</au><au>YAN HUAYUAN</au><au>ZHENG WANGJIAN</au><au>CHEN XIAOLONG</au><au>JIA XIAOQIN</au><au>HOU QIANGBO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Recombinant genetic engineering bacterium and application thereof in production of vanillin by fermentation</title><date>2019-09-20</date><risdate>2019</risdate><abstract>The invention discloses a recombinant genetic engineering bacterium and an application thereof in production of vanillin by fermentation. The recombinant genetic engineering bacterium is constructed by introducing a gene tolerant to vanillin or ferulic acid into a host bacterium. The gene tolerant to vanillin or ferulic acid includes one or more of a periplasmic membrane fusion protein AcrA gene, an inner membrane transport protein AcrB gene or an outer membrane protein TolC gene; the host includes escherichia coli containing an ech gene and an fcs gene. The recombinant genetic engineering bacterium E.coli BW25113::PegYB::pACYCDuet-acrAB-tolC can produce vanillin with ferulic acid as a substrate, and the maximum yield of vanillin is 2335 mg/L. A fermentation broth produced by fermentation by the method contains higher vanillin and good application prospect is achieved.
本发明公开了一种重组基因工程菌及其发酵产香兰素的应用，所述重组基因工程菌是由耐受香兰素或阿魏酸的基因导入宿主菌构建而成，所述耐受香兰素或阿魏酸的基因包括周质膜融合蛋白AcrA基因、内膜转运蛋白AcrB基因或外膜蛋白TolC基因中的一种或多种；所述宿主包括含ech基因和fcs基因的大肠</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Recombinant genetic engineering bacterium and application thereof in production of vanillin by fermentation |
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