Synthetic culture medium for high yield of chitinase through serratia marcescens and fermentation method of synthetic culture medium

The invention provides a synthetic culture medium for high yield of chitinase through serratia marcescens and a fermentation method of the synthetic culture medium, and belongs to the technical field of microorganisms. The synthetic culture medium comprises 3 g of beef paste, 10 g of cane sugar, 5 g...

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Hauptverfasser: WEI YIBIN, WANG ZHIQING, HUANG SILIANG, TAO AILI, PANG FAHU, XU RUXIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a synthetic culture medium for high yield of chitinase through serratia marcescens and a fermentation method of the synthetic culture medium, and belongs to the technical field of microorganisms. The synthetic culture medium comprises 3 g of beef paste, 10 g of cane sugar, 5 g of a nitrogen source, 10 g of NaCl, 15 g of agar powder and 1000 mL of distilled water. The preparation method comprises the steps that 3 g of the beef paste, 10 g of the cane sugar, 5 g of the nitrogen source, 10 g of NaCl and 15 g of the agar powder are added, 1000 mL of the distilled water is added, and the mixture is heated to be thoroughly melted, subpackaged into a conical flask and sterilized at 121 DEG C. The requirements of the market for chitinase are reduced, the preparation cost is low, the preparation process is simple and environmentally friendly, and the utilization rate of the serratia marcescens TC-1 is also improved. 本发明提供一种粘质沙雷氏菌高产几丁质酶的合成培养基及其发酵方法,属于微生物技术领域,合成培养基的配方为:牛肉膏3 g,蔗糖10 g,氮源5 g,NaCL10 g