CELL CRYOPRESERVATION COMPOSITION AND CRYOPRESERVATION METHOD

The present invention is added by a simple means when cells are to be cryopreserved and improves the survival rate of the cells. According to the present invention, cells are stored after 1 vol% of acomposition containing 0.01-20 wt% of sophorose lipid is added thereto during a period between six hours before and immediately before cryopreservation of the cells. This composition is capable of reducing frost damage to cells, and thereby improves cell survival rate and enables storage of cells without using DMSO and blood serum. In addition, the present invention provides a simple and inexpensive storage method that does not require delicate control on freezing speed during cell cryopreservation. 冻存细胞时，简便地进行添加来提高细胞的存活率。一种组合物，含有0.01重量％到20重量％的槐糖脂，从即将冻存细胞到冻存细胞6小时前的期间，添加该组合物并使该组合物达到1容量％后保存细胞。该组合物会减少细胞遭受的冻害，由此可提高冻存时的细胞存活率，从而能够在不使用DMSO和血清的情况下保存细胞。并且，冻存细胞时也不需要对冻结速率进行精细的控制，是一种简便低廉的保存方法。