Method for separating and measuring contents of various components in Huoxiang Zhengqi oral liquid by HPLC method

The invention belongs to the field of pharmaceutical preparations, relates to a quality control method of Huoxiang Zhengqi oral liquid, and in particular relates to a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. The inve...

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Hauptverfasser: QIN SHAORONG, CHEN JINFENG, PENG TAO, DONG ZILIANG, DENG YUN, GUO DALE, LI ZHANGYU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of pharmaceutical preparations, relates to a quality control method of Huoxiang Zhengqi oral liquid, and in particular relates to a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. The invention provides a method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by an HPLC method. According to the HPLC method, a chromatographic column is adopted, and a silica gel matrix is taken as a filler, and a solution A and a solution B are mixed according to a certain proportion to be taken as a mobile phase for elution; the solution A is acetonitrile, and the solution B is phosphoric acid water; and the components comprise one or more of licocoumarin, isoliquiritigenin, apigenin liquiritin, naringin, liquiritin and nobiletin. The method for separating and measuring the content of various components in the Huoxiang Zhengqi oral liquid by the HPLC method is simple