African swine fever virus tandem gene, coexpression vector, construction method and application
The invention belongs to the technical field of genetic engineering vaccines, and particularly relates to an African swine fever virus tandem gene, a coexpression vector, a construction method and application. The recombinant vector is obtained through the steps that an artificially synthesized poly...
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| creator | ZHAO WEN YUE CHANGWU SUN XINYUE LYU YUHONG CUI XIANGYI DANG DONGMEI TIAN HONGYING |
| description | The invention belongs to the technical field of genetic engineering vaccines, and particularly relates to an African swine fever virus tandem gene, a coexpression vector, a construction method and application. The recombinant vector is obtained through the steps that an artificially synthesized polygene clone expression element is connected to the portion between the BglII and BamHI restriction enzyme cutting sites in a pIRES2-egfp vector, and oriR101 is inserted in the portion behind the XbaI site of 1986 nt of the pIRES2-egfp vector, wherein the nucleotide sequence of the gene clone expression element is shown in SEQ ID NO: 1; the recombinant vector is transformed into escherichia coli competent cells, screening and cultivation enlargement are conducted, then polygenic protein is collected and purified, and a subunit vaccine against the African swine fever disease is obtained; the recombinant vector is transformed into lactobacillus competent cells, and after screening and cultivation enlargement are conduc |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | African swine fever virus tandem gene, coexpression vector, construction method and application |
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