Two gRNA sequences capable of targeting two genes xa13 and xa25 at same time and efficiently creating bacterial-blight-resistant rice
The invention discloses a base sequence 5'-TCGGTGCCGTACGTGGTGGCGCTCTTCAGC-3' of 30bp in length. The sequence is a long homologous sequence unique to rice bacterial blight infected genes xa13 and xa25,and the sequence is utilized to disclose two gRNAs capable of knocking out xa13 and xa25 i...
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| creator | GUO XINRUI CHEN JIANMIN ZHU YIWANG LIN YARONG ZHOU JUN'AI MEI FATING CHEN RUI WANG FENG |
| description | The invention discloses a base sequence 5'-TCGGTGCCGTACGTGGTGGCGCTCTTCAGC-3' of 30bp in length. The sequence is a long homologous sequence unique to rice bacterial blight infected genes xa13 and xa25,and the sequence is utilized to disclose two gRNAs capable of knocking out xa13 and xa25 infected genes in a targeted manner at the same time. Broad-spectrum rice new materials with double gene mutation and highly resistant to bacterial blight can be screened and obtained by utilizing the gRNAs, an efficient breeding mode is provided for creating broad-spectrum lasting disease-resistant materials, and the sequence is of important significance in studying prevention and control of rice bacterial blight.
本发明公开了一段长度为30bp的碱基序列5'- TCGGTGCCGTACGTGGTGGCGCTCTTCAGC -3'，该序列是水稻白叶枯感病基因xa13与xa25唯一一段较长的同源序列，并利用该序列公开了两个可以同时靶向敲除xa13与xa25感病基因的gRNA。利用该gRNA可以筛选并获得双基因突变的广谱高抗白叶枯的水稻新材料，该发明为广谱持久抗病材料的创制提供一种高效的育种方式，同时对水稻白叶枯病的防治研究具有重要的意义。 |
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本发明公开了一段长度为30bp的碱基序列5'- TCGGTGCCGTACGTGGTGGCGCTCTTCAGC -3'，该序列是水稻白叶枯感病基因xa13与xa25唯一一段较长的同源序列，并利用该序列公开了两个可以同时靶向敲除xa13与xa25感病基因的gRNA。利用该gRNA可以筛选并获得双基因突变的广谱高抗白叶枯的水稻新材料，该发明为广谱持久抗病材料的创制提供一种高效的育种方式，同时对水稻白叶枯病的防治研究具有重要的意义。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190816&DB=EPODOC&CC=CN&NR=110129325A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190816&DB=EPODOC&CC=CN&NR=110129325A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GUO XINRUI</creatorcontrib><creatorcontrib>CHEN JIANMIN</creatorcontrib><creatorcontrib>ZHU YIWANG</creatorcontrib><creatorcontrib>LIN YARONG</creatorcontrib><creatorcontrib>ZHOU JUN'AI</creatorcontrib><creatorcontrib>MEI FATING</creatorcontrib><creatorcontrib>CHEN RUI</creatorcontrib><creatorcontrib>WANG FENG</creatorcontrib><title>Two gRNA sequences capable of targeting two genes xa13 and xa25 at same time and efficiently creating bacterial-blight-resistant rice</title><description>The invention discloses a base sequence 5'-TCGGTGCCGTACGTGGTGGCGCTCTTCAGC-3' of 30bp in length. The sequence is a long homologous sequence unique to rice bacterial blight infected genes xa13 and xa25,and the sequence is utilized to disclose two gRNAs capable of knocking out xa13 and xa25 infected genes in a targeted manner at the same time. Broad-spectrum rice new materials with double gene mutation and highly resistant to bacterial blight can be screened and obtained by utilizing the gRNAs, an efficient breeding mode is provided for creating broad-spectrum lasting disease-resistant materials, and the sequence is of important significance in studying prevention and control of rice bacterial blight.
本发明公开了一段长度为30bp的碱基序列5'- TCGGTGCCGTACGTGGTGGCGCTCTTCAGC -3'，该序列是水稻白叶枯感病基因xa13与xa25唯一一段较长的同源序列，并利用该序列公开了两个可以同时靶向敲除xa13与xa25感病基因的gRNA。利用该gRNA可以筛选并获得双基因突变的广谱高抗白叶枯的水稻新材料，该发明为广谱持久抗病材料的创制提供一种高效的育种方式，同时对水稻白叶枯病的防治研究具有重要的意义。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNTUsKwjAU7MaFqHd4HqBgWly4LEVx1YV0X17jS3wQ05o8UQ_gvU3FA7iZGebDzLN3-xjAnpoKIt3u5DVF0Dhi7wgGA4LBkrC3IFOPfIqfqEpAf06i2AIKRLwSCCeYXDKGNZMX9wIdCL_rHrVQYHR579heJA8UOQp6gcCaltnMoIu0-vEiWx_2bX3MaRw6iiPq9Cxd3Si1UcWuLLZV-U_nA7l3SZ8</recordid><startdate>20190816</startdate><enddate>20190816</enddate><creator>GUO XINRUI</creator><creator>CHEN JIANMIN</creator><creator>ZHU YIWANG</creator><creator>LIN YARONG</creator><creator>ZHOU JUN'AI</creator><creator>MEI FATING</creator><creator>CHEN RUI</creator><creator>WANG FENG</creator><scope>EVB</scope></search><sort><creationdate>20190816</creationdate><title>Two gRNA sequences capable of targeting two genes xa13 and xa25 at same time and efficiently creating bacterial-blight-resistant rice</title><author>GUO XINRUI ; CHEN JIANMIN ; ZHU YIWANG ; LIN YARONG ; ZHOU JUN'AI ; MEI FATING ; CHEN RUI ; WANG FENG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN110129325A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GUO XINRUI</creatorcontrib><creatorcontrib>CHEN JIANMIN</creatorcontrib><creatorcontrib>ZHU YIWANG</creatorcontrib><creatorcontrib>LIN YARONG</creatorcontrib><creatorcontrib>ZHOU JUN'AI</creatorcontrib><creatorcontrib>MEI FATING</creatorcontrib><creatorcontrib>CHEN RUI</creatorcontrib><creatorcontrib>WANG FENG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GUO XINRUI</au><au>CHEN JIANMIN</au><au>ZHU YIWANG</au><au>LIN YARONG</au><au>ZHOU JUN'AI</au><au>MEI FATING</au><au>CHEN RUI</au><au>WANG FENG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Two gRNA sequences capable of targeting two genes xa13 and xa25 at same time and efficiently creating bacterial-blight-resistant rice</title><date>2019-08-16</date><risdate>2019</risdate><abstract>The invention discloses a base sequence 5'-TCGGTGCCGTACGTGGTGGCGCTCTTCAGC-3' of 30bp in length. The sequence is a long homologous sequence unique to rice bacterial blight infected genes xa13 and xa25,and the sequence is utilized to disclose two gRNAs capable of knocking out xa13 and xa25 infected genes in a targeted manner at the same time. Broad-spectrum rice new materials with double gene mutation and highly resistant to bacterial blight can be screened and obtained by utilizing the gRNAs, an efficient breeding mode is provided for creating broad-spectrum lasting disease-resistant materials, and the sequence is of important significance in studying prevention and control of rice bacterial blight.
本发明公开了一段长度为30bp的碱基序列5'- TCGGTGCCGTACGTGGTGGCGCTCTTCAGC -3'，该序列是水稻白叶枯感病基因xa13与xa25唯一一段较长的同源序列，并利用该序列公开了两个可以同时靶向敲除xa13与xa25感病基因的gRNA。利用该gRNA可以筛选并获得双基因突变的广谱高抗白叶枯的水稻新材料，该发明为广谱持久抗病材料的创制提供一种高效的育种方式，同时对水稻白叶枯病的防治研究具有重要的意义。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Two gRNA sequences capable of targeting two genes xa13 and xa25 at same time and efficiently creating bacterial-blight-resistant rice |
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