Method for improving bovine somatic cell cloning efficiency by inducing expression of zfp57

The invention discloses a method for improving bovine somatic cell cloning efficiency. By inducing the expression of zfp57 gene, the methylation of imprinted genes in the development process of a bovine cloned embryo is restored to a normal level, so that the cloning efficiency of bovine somatic cells is improved. The method for improving the bovine somatic cell cloning efficiency provided by theinvention utilizes the zfp57 to correct the abnormal low methylation of imprinted genes on the bovine cloned embryo, and makes the methylation level close to that of in vitro fertilization embryos soas to improve the embryo quality of the bovine cloned embryos, and to promote the development of the bovine cloned embryos. The method provides a theoretical basis for clarifying the mechanism of abnormal methylation imprinting in the process of reprogramming somatic cell-cloned embryos, and the reason of low efficiency of somatic cell cloning. 本发明公开了一种提高牛体细胞克隆效率的方法，通过诱导表达zfp57基因使牛克隆胚胎发育过程中的印迹基因甲基化恢复正常水平，从而提高牛体细胞克隆效率。本发明提供