Method for obtaining sweat gland organs from separation and culture of sweat gland cells and application thereof

The invention discloses a method for obtaining sweet gland organs from separation and culture of sweat gland cells and application thereof, and belongs to the technical field of biological engineering. According to the sweat gland cell separation method, multi-step separation is performed by adopting combination of digestive enzymes, and sweat gland cells can be efficiently separated. The separated sweat gland cells contain dry cells, the cells can be used for culturing a three-dimensional culture medium, the sweat gland organs with the high cell activity and two-way differentiation potentialare obtained, the organs are transplanted to mice with damage to whole sole and back, regeneration and function recovery of sweat glands can be promoted, skin wound healing can be promoted, and the method can be used for preparing cell products for repairing skin damage and recovering auxiliary organ functions, and the application prospect is wide. 本发明公开一种汗腺细胞的分离和培养获得汗腺类器官的方法及其应用，属于生物工程技术领域，其中所述汗腺细胞的分离方法采用消化酶的组合进行多步分离，可以实