Detection method of amylase

The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: YAO HUAN, FU CUIXIA, YUE XULIANG, SU HAITAO, LI JIANLEI, YU WENHUA, LIU DOU, KANG ZHIYUAN, LIU FENGZAN, WANG HONGYE, YANG XINYAO, WANG SHAOBO, ZHANG GUANGHUI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator YAO HUAN
FU CUIXIA
YUE XULIANG
SU HAITAO
LI JIANLEI
YU WENHUA
LIU DOU
KANG ZHIYUAN
LIU FENGZAN
WANG HONGYE
YANG XINYAO
WANG SHAOBO
ZHANG GUANGHUI
description The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granules is observed under a microscope, and morphological changes of the starch granules are used to determine whether the sample to be detected contains the amylase. If the starch granules swell significantly, become lighter in color or disappear, the sample to be detected contains the amylase. If themorphological changes of the starch granules are not shown, the sample to be detected is free of the amylase. The provided detection method is simple and convenient to operate, intuitive and easy tounderstand in results, high in detection precision, simple in sample processing and small in required sample amount. 本发明公开了种淀粉酶的检测方法,该检测方法的原理是以待测样品和淀粉混合后溶解在水中,用碘化钾-碘溶液染色,在显微镜下观察淀粉颗粒的形态,通过淀粉颗粒的形态变化判断待测样品中有无淀粉酶。若淀粉颗粒明显膨胀、颜色变浅或颗粒消
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN109536575A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN109536575A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN109536575A3</originalsourceid><addsrcrecordid>eNrjZJB2SS1JTS7JzM9TyE0tychPUchPU0jMrcxJLE7lYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBpamxmam5qaOxsSoAQBrRiNV</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Detection method of amylase</title><source>esp@cenet</source><creator>YAO HUAN ; FU CUIXIA ; YUE XULIANG ; SU HAITAO ; LI JIANLEI ; YU WENHUA ; LIU DOU ; KANG ZHIYUAN ; LIU FENGZAN ; WANG HONGYE ; YANG XINYAO ; WANG SHAOBO ; ZHANG GUANGHUI</creator><creatorcontrib>YAO HUAN ; FU CUIXIA ; YUE XULIANG ; SU HAITAO ; LI JIANLEI ; YU WENHUA ; LIU DOU ; KANG ZHIYUAN ; LIU FENGZAN ; WANG HONGYE ; YANG XINYAO ; WANG SHAOBO ; ZHANG GUANGHUI</creatorcontrib><description>The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granules is observed under a microscope, and morphological changes of the starch granules are used to determine whether the sample to be detected contains the amylase. If the starch granules swell significantly, become lighter in color or disappear, the sample to be detected contains the amylase. If themorphological changes of the starch granules are not shown, the sample to be detected is free of the amylase. The provided detection method is simple and convenient to operate, intuitive and easy tounderstand in results, high in detection precision, simple in sample processing and small in required sample amount. 本发明公开了种淀粉酶的检测方法,该检测方法的原理是以待测样品和淀粉混合后溶解在水中,用碘化钾-碘溶液染色,在显微镜下观察淀粉颗粒的形态,通过淀粉颗粒的形态变化判断待测样品中有无淀粉酶。若淀粉颗粒明显膨胀、颜色变浅或颗粒消</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20190329&amp;DB=EPODOC&amp;CC=CN&amp;NR=109536575A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20190329&amp;DB=EPODOC&amp;CC=CN&amp;NR=109536575A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YAO HUAN</creatorcontrib><creatorcontrib>FU CUIXIA</creatorcontrib><creatorcontrib>YUE XULIANG</creatorcontrib><creatorcontrib>SU HAITAO</creatorcontrib><creatorcontrib>LI JIANLEI</creatorcontrib><creatorcontrib>YU WENHUA</creatorcontrib><creatorcontrib>LIU DOU</creatorcontrib><creatorcontrib>KANG ZHIYUAN</creatorcontrib><creatorcontrib>LIU FENGZAN</creatorcontrib><creatorcontrib>WANG HONGYE</creatorcontrib><creatorcontrib>YANG XINYAO</creatorcontrib><creatorcontrib>WANG SHAOBO</creatorcontrib><creatorcontrib>ZHANG GUANGHUI</creatorcontrib><title>Detection method of amylase</title><description>The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granules is observed under a microscope, and morphological changes of the starch granules are used to determine whether the sample to be detected contains the amylase. If the starch granules swell significantly, become lighter in color or disappear, the sample to be detected contains the amylase. If themorphological changes of the starch granules are not shown, the sample to be detected is free of the amylase. The provided detection method is simple and convenient to operate, intuitive and easy tounderstand in results, high in detection precision, simple in sample processing and small in required sample amount. 本发明公开了种淀粉酶的检测方法,该检测方法的原理是以待测样品和淀粉混合后溶解在水中,用碘化钾-碘溶液染色,在显微镜下观察淀粉颗粒的形态,通过淀粉颗粒的形态变化判断待测样品中有无淀粉酶。若淀粉颗粒明显膨胀、颜色变浅或颗粒消</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJB2SS1JTS7JzM9TyE0tychPUchPU0jMrcxJLE7lYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBpamxmam5qaOxsSoAQBrRiNV</recordid><startdate>20190329</startdate><enddate>20190329</enddate><creator>YAO HUAN</creator><creator>FU CUIXIA</creator><creator>YUE XULIANG</creator><creator>SU HAITAO</creator><creator>LI JIANLEI</creator><creator>YU WENHUA</creator><creator>LIU DOU</creator><creator>KANG ZHIYUAN</creator><creator>LIU FENGZAN</creator><creator>WANG HONGYE</creator><creator>YANG XINYAO</creator><creator>WANG SHAOBO</creator><creator>ZHANG GUANGHUI</creator><scope>EVB</scope></search><sort><creationdate>20190329</creationdate><title>Detection method of amylase</title><author>YAO HUAN ; FU CUIXIA ; YUE XULIANG ; SU HAITAO ; LI JIANLEI ; YU WENHUA ; LIU DOU ; KANG ZHIYUAN ; LIU FENGZAN ; WANG HONGYE ; YANG XINYAO ; WANG SHAOBO ; ZHANG GUANGHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109536575A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YAO HUAN</creatorcontrib><creatorcontrib>FU CUIXIA</creatorcontrib><creatorcontrib>YUE XULIANG</creatorcontrib><creatorcontrib>SU HAITAO</creatorcontrib><creatorcontrib>LI JIANLEI</creatorcontrib><creatorcontrib>YU WENHUA</creatorcontrib><creatorcontrib>LIU DOU</creatorcontrib><creatorcontrib>KANG ZHIYUAN</creatorcontrib><creatorcontrib>LIU FENGZAN</creatorcontrib><creatorcontrib>WANG HONGYE</creatorcontrib><creatorcontrib>YANG XINYAO</creatorcontrib><creatorcontrib>WANG SHAOBO</creatorcontrib><creatorcontrib>ZHANG GUANGHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YAO HUAN</au><au>FU CUIXIA</au><au>YUE XULIANG</au><au>SU HAITAO</au><au>LI JIANLEI</au><au>YU WENHUA</au><au>LIU DOU</au><au>KANG ZHIYUAN</au><au>LIU FENGZAN</au><au>WANG HONGYE</au><au>YANG XINYAO</au><au>WANG SHAOBO</au><au>ZHANG GUANGHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Detection method of amylase</title><date>2019-03-29</date><risdate>2019</risdate><abstract>The present invention discloses a detection method of amylase. A principle of the detection method is as follows: after a sample to be detected and starch are mixed, the mixture is dissolved in water,the dissolved mixture is stained with a potassium iodide-iodine solution, morphology of starch granules is observed under a microscope, and morphological changes of the starch granules are used to determine whether the sample to be detected contains the amylase. If the starch granules swell significantly, become lighter in color or disappear, the sample to be detected contains the amylase. If themorphological changes of the starch granules are not shown, the sample to be detected is free of the amylase. The provided detection method is simple and convenient to operate, intuitive and easy tounderstand in results, high in detection precision, simple in sample processing and small in required sample amount. 本发明公开了种淀粉酶的检测方法,该检测方法的原理是以待测样品和淀粉混合后溶解在水中,用碘化钾-碘溶液染色,在显微镜下观察淀粉颗粒的形态,通过淀粉颗粒的形态变化判断待测样品中有无淀粉酶。若淀粉颗粒明显膨胀、颜色变浅或颗粒消</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN109536575A
source esp@cenet
subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title Detection method of amylase
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T21%3A46%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YAO%20HUAN&rft.date=2019-03-29&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN109536575A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true