Method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis
The invention discloses a method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis, and belongs to the technical field of biological engineering.The method successfully realizes the co-expression building of phenylalanine dehydrogenase and lactat...
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| creator | XUE JIKE WANG MIAO LIU LONG |
| description | The invention discloses a method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis, and belongs to the technical field of biological engineering.The method successfully realizes the co-expression building of phenylalanine dehydrogenase and lactate dehydrogenase in bacillus subtilis. The whole cell transformation of L-phenylalanine can be usedfor producing 10.0 g/L phenyllactic acid; the transformation rate is 40 percent. Through the building of the whole cell transformation system, the problems of complicated steps, low yield, environmentpollution and the like of phenyllactic acid transformation by a chemical method can be solved; a certain theory basis is laid for the subsequent industrialized production.
本发明公开了种枯草芽孢杆菌全细胞转化苯丙氨酸生产苯乳酸的方法，属于生物工程技术领域。本发明成功实现了苯丙氨酸脱氢酶、乳酸脱氢酶在枯草芽孢杆菌中共表达的构建。全细胞转化L-苯丙氨酸可生产10.0g/L苯乳酸，转化率为40％。此全细胞转化体系的建立，解决了化学法合成苯乳酸的步骤繁琐、得率低、污染环境等问题，为后续工业化生产奠定了定的理论基础。 |
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本发明公开了种枯草芽孢杆菌全细胞转化苯丙氨酸生产苯乳酸的方法，属于生物工程技术领域。本发明成功实现了苯丙氨酸脱氢酶、乳酸脱氢酶在枯草芽孢杆菌中共表达的构建。全细胞转化L-苯丙氨酸可生产10.0g/L苯乳酸，转化率为40％。此全细胞转化体系的建立，解决了化学法合成苯乳酸的步骤繁琐、得率低、污染环境等问题，为后续工业化生产奠定了定的理论基础。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190326&DB=EPODOC&CC=CN&NR=109517778A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190326&DB=EPODOC&CC=CN&NR=109517778A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XUE JIKE</creatorcontrib><creatorcontrib>WANG MIAO</creatorcontrib><creatorcontrib>LIU LONG</creatorcontrib><title>Method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis</title><description>The invention discloses a method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis, and belongs to the technical field of biological engineering.The method successfully realizes the co-expression building of phenylalanine dehydrogenase and lactate dehydrogenase in bacillus subtilis. The whole cell transformation of L-phenylalanine can be usedfor producing 10.0 g/L phenyllactic acid; the transformation rate is 40 percent. Through the building of the whole cell transformation system, the problems of complicated steps, low yield, environmentpollution and the like of phenyllactic acid transformation by a chemical method can be solved; a certain theory basis is laid for the subsequent industrialized production.
本发明公开了种枯草芽孢杆菌全细胞转化苯丙氨酸生产苯乳酸的方法，属于生物工程技术领域。本发明成功实现了苯丙氨酸脱氢酶、乳酸脱氢酶在枯草芽孢杆菌中共表达的构建。全细胞转化L-苯丙氨酸可生产10.0g/L苯乳酸，转化率为40％。此全细胞转化体系的建立，解决了化学法合成苯乳酸的步骤繁琐、得率低、污染环境等问题，为后续工业化生产奠定了定的理论基础。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyz0KwkAQQOE0FqLeYTyAYBCJKSUoNlrZh8lmkl0YZ5b9QXJ7U-QAVq9537qQJyWrPQwawAfts3EygrckEzOa5AygcT0kGzSPFr5WmcAQM6SAEmf3weRUQIeFIaM4Iegm6GbLnCPE3CXHLm6L1YAcabd0U-zvt3fzOJDXlqJHQ0KpbV7lsT6XVVVdrqd_nh88C0Og</recordid><startdate>20190326</startdate><enddate>20190326</enddate><creator>XUE JIKE</creator><creator>WANG MIAO</creator><creator>LIU LONG</creator><scope>EVB</scope></search><sort><creationdate>20190326</creationdate><title>Method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis</title><author>XUE JIKE ; WANG MIAO ; LIU LONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109517778A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>XUE JIKE</creatorcontrib><creatorcontrib>WANG MIAO</creatorcontrib><creatorcontrib>LIU LONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XUE JIKE</au><au>WANG MIAO</au><au>LIU LONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis</title><date>2019-03-26</date><risdate>2019</risdate><abstract>The invention discloses a method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis, and belongs to the technical field of biological engineering.The method successfully realizes the co-expression building of phenylalanine dehydrogenase and lactate dehydrogenase in bacillus subtilis. The whole cell transformation of L-phenylalanine can be usedfor producing 10.0 g/L phenyllactic acid; the transformation rate is 40 percent. Through the building of the whole cell transformation system, the problems of complicated steps, low yield, environmentpollution and the like of phenyllactic acid transformation by a chemical method can be solved; a certain theory basis is laid for the subsequent industrialized production.
本发明公开了种枯草芽孢杆菌全细胞转化苯丙氨酸生产苯乳酸的方法，属于生物工程技术领域。本发明成功实现了苯丙氨酸脱氢酶、乳酸脱氢酶在枯草芽孢杆菌中共表达的构建。全细胞转化L-苯丙氨酸可生产10.0g/L苯乳酸，转化率为40％。此全细胞转化体系的建立，解决了化学法合成苯乳酸的步骤繁琐、得率低、污染环境等问题，为后续工业化生产奠定了定的理论基础。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for producing phenyllactic acid through whole cell transformation of phenylalanine by bacillus subtilis |
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