Single cell agarose gel electrophoresis kit

Single cell agarose gel electrophoresis kit

The invention relates to the field of biotechnology, specifically, relates to a single cell agarose gel electrophoresis kit. The invention consists of the following reagents: electrophoresis reagent 40 ~ 50ml, silver dye reagent 40 ~ 60ml, and fixing adhesive 80 ~ 130ul. The electrophoresis reagent...

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1. Verfasser: JIN YUKUAN
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Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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description The invention relates to the field of biotechnology, specifically, relates to a single cell agarose gel electrophoresis kit. The invention consists of the following reagents: electrophoresis reagent 40 ~ 50ml, silver dye reagent 40 ~ 60ml, and fixing adhesive 80 ~ 130ul. The electrophoresis reagent comprises 30-56 ml of LS lysate, 2-10 ml of LMA agar, special slide glass for TC comet electrophoresis and 0.5-2 mM of EDTA; By using the electrophoresis kit in the comet assay, fragments (the comet tails) that migrate from the nucleus (the comet head) can be observed. Negatively charged DNA migrates toward the anode, and the length of extrusion reflects the increased relaxation of the supercoil. The common description of DNA damage in the comet assay is the percentage of DNA on the tail and thecomet tail time. The percentage of DNA on the tail is a standardized measure of the percentage of total cellular DNA on the tail. The single cell agarose gel electrophoresis kit is easy to generate quantitative and statistic
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Single cell agarose gel electrophoresis kit
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