Preparation method of polypeptide mixed concentrate

The invention discloses a preparation method of polypeptide mixed concentrate. The preparation method comprises the following three steps: firstly, separating umbilical cord Wharton's jelly; secondly,separating mesenchymal stem cells; thirdly, extracting high-purity polypeptide mixed concentrat...

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Hauptverfasser: LU YI, XIANG SHENGQUN, LI JUNXIANG, DONG YUNJIA, YU JIANFENG, ZHOU BILIU, SHEN YUNXIA, MAO SHUHUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method of polypeptide mixed concentrate. The preparation method comprises the following three steps: firstly, separating umbilical cord Wharton's jelly; secondly,separating mesenchymal stem cells; thirdly, extracting high-purity polypeptide mixed concentrate. Compared with a preparation method in the prior art, the preparation method of the polypeptide mixedconcentrate, disclosed by the invention, has the beneficial effects that the Wharton's jelly can be efficiently separated, and the mesenchymal stem cells with high purity and high vitality can be separated and purified from the Wharton's jelly; amplification subculture is continuously carried out, and thereby the polypeptide mixed concentrate is collected in a high-speed proliferation period of stem cells. 本发明公开了种多肽混合原液的制备方法,包括三个步骤:、脐带华通氏胶的分离;二、分离间充质干细胞;三、提取高纯度的多肽混合原液;本发明的多肽混合原液的制备方法对比现有技术中的制备方法具备下述有益效果:能够高效分离出华通氏胶,然后从华通氏胶中分离纯化出高纯度高活力的间充质干细胞,继续扩增传代培养,从而在干细胞高速增殖期收集提取多肽混合原液。