Mixed fermentation enzyme production technology

The invention belongs to the technical field of fermentation and discloses a mixed fermentation enzyme production technology. The technology comprises the steps as follows: a fermentation tank is inoculated with a trichoderma reesei seed solution with the inoculation amount being 5%-10%, culture is performed for 12 h, then, the fermentation tank is inoculated with a rhizopus stolonifer seed solution and a bacillus pumilus seed solution with the inoculation amount both being 5%-10%, culture is performed continuously for 12 h, then, corn straw powder is added, culture is performed continuously for 48-60 h, and a fermentation broth is obtained. By means of the strain mixed fermentation enzyme production method, the synergistic effect is good and the enzyme production efficiency is high. 本发明属于发酵技术领域，公开了种混合发酵产酶工艺，其包括如下步骤：将里氏木霉种子液按照5-10%的接种量接种到发酵罐中，培养12h，然后接种匍枝根霉种子液和短小芽孢杆菌种子液，接种量均为5-10%，继续培养12h，然后添加玉米秸秆粉，继续培养48-60h，得到发酵液。本发明采用菌株混合发酵产酶方式，协同效果好，产酶效率高。