Method for rapidly determining genome size of sturgeons by flow cytometry
The invention discloses a method for rapidly determining the genome size sturgeons of by flow cytometry. The method comprises the following steps: 1, selecting fish with the genome having a known sizeas a standard control, respectively performing caudal vein blood connection, blood centrifuging, PBS...
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| creator | ZHENG RONGQUAN MEI YIYUN WANG ZHIGANG |
| description | The invention discloses a method for rapidly determining the genome size sturgeons of by flow cytometry. The method comprises the following steps: 1, selecting fish with the genome having a known sizeas a standard control, respectively performing caudal vein blood connection, blood centrifuging, PBS buffer solution washing, 2% paraformaldehyde solution addition, permeabilizing agent addition andstaining on the standard control and a sturgeon to be detected, and detecting the standard control and the sturgeon to be detected by flow cytometry to obtain the fluorescence intensity peak P2 of thestandard control and the fluorescence intensity peak P1 of the sturgeon to be detected; and 2, obtaining the genome size of the sturgeon to be detected according to N1= N2 * P1/P2, wherein N1 represents the genome size of the sturgeon to be detected, and N2 represents the genome size of the standard control. The method can be used to preliminary determine the genome size of several major speciesof sturgeons bred in China b |
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The method comprises the following steps: 1, selecting fish with the genome having a known sizeas a standard control, respectively performing caudal vein blood connection, blood centrifuging, PBS buffer solution washing, 2% paraformaldehyde solution addition, permeabilizing agent addition andstaining on the standard control and a sturgeon to be detected, and detecting the standard control and the sturgeon to be detected by flow cytometry to obtain the fluorescence intensity peak P2 of thestandard control and the fluorescence intensity peak P1 of the sturgeon to be detected; and 2, obtaining the genome size of the sturgeon to be detected according to N1= N2 * P1/P2, wherein N1 represents the genome size of the sturgeon to be detected, and N2 represents the genome size of the standard control. 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The method comprises the following steps: 1, selecting fish with the genome having a known sizeas a standard control, respectively performing caudal vein blood connection, blood centrifuging, PBS buffer solution washing, 2% paraformaldehyde solution addition, permeabilizing agent addition andstaining on the standard control and a sturgeon to be detected, and detecting the standard control and the sturgeon to be detected by flow cytometry to obtain the fluorescence intensity peak P2 of thestandard control and the fluorescence intensity peak P1 of the sturgeon to be detected; and 2, obtaining the genome size of the sturgeon to be detected according to N1= N2 * P1/P2, wherein N1 represents the genome size of the sturgeon to be detected, and N2 represents the genome size of the standard control. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Method for rapidly determining genome size of sturgeons by flow cytometry |
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