Reagent kit and method for detecting DNA (deoxyribonucleic acid) methylation and application
The invention discloses a reagent kit for detecting DNA (deoxyribonucleic acid) methylation. The reagent kit at least comprises biotin-labeled methylated lambda-DNA and streptomycin-enveloped magneticbeads. The invention further discloses application of the reagent kit to detecting methylation of a...
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| creator | MAO ZHANRUI JIN GEXUAN SHAO ZHIFENG KANG YANI ZHAO XIAODONG |
| description | The invention discloses a reagent kit for detecting DNA (deoxyribonucleic acid) methylation. The reagent kit at least comprises biotin-labeled methylated lambda-DNA and streptomycin-enveloped magneticbeads. The invention further discloses application of the reagent kit to detecting methylation of a few DNA samples and a method for detecting the DNA methylation and application of the method. The low sample amount can reach 1 ng. According to the technical scheme, the reagent kit, the application and the method have the advantages that the lowermost initial sample amount required for detectingthe DNA methylation can be obviously lowered, specific reagent kits and experimental equipment can be omitted, accordingly, the cost can be saved, and the reagent kit and the method are high in universality; exogenous DNA pollution can be prevented when methylated DNA is enriched, influence of exogenous DNA information can be prevented if processes for detecting the DNA methylation by means of library construction and high |
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The reagent kit at least comprises biotin-labeled methylated lambda-DNA and streptomycin-enveloped magneticbeads. The invention further discloses application of the reagent kit to detecting methylation of a few DNA samples and a method for detecting the DNA methylation and application of the method. The low sample amount can reach 1 ng. According to the technical scheme, the reagent kit, the application and the method have the advantages that the lowermost initial sample amount required for detectingthe DNA methylation can be obviously lowered, specific reagent kits and experimental equipment can be omitted, accordingly, the cost can be saved, and the reagent kit and the method are high in universality; exogenous DNA pollution can be prevented when methylated DNA is enriched, influence of exogenous DNA information can be prevented if processes for detecting the DNA methylation by means of library construction and high</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181113&DB=EPODOC&CC=CN&NR=108796039A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20181113&DB=EPODOC&CC=CN&NR=108796039A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MAO ZHANRUI</creatorcontrib><creatorcontrib>JIN GEXUAN</creatorcontrib><creatorcontrib>SHAO ZHIFENG</creatorcontrib><creatorcontrib>KANG YANI</creatorcontrib><creatorcontrib>ZHAO XIAODONG</creatorcontrib><title>Reagent kit and method for detecting DNA (deoxyribonucleic acid) methylation and application</title><description>The invention discloses a reagent kit for detecting DNA (deoxyribonucleic acid) methylation. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Reagent kit and method for detecting DNA (deoxyribonucleic acid) methylation and application |
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